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Preliminary photosynthesis measurements of selected wheat cultivars prior to inoculation. Dr. Ferenc Horváth Dr. Barnabás Wodala. The LI-6400 Portable Photosynthesis System. The LI-6400 Portable Photosynthesis System. The LI-6400 Portable Photosynthesis System. An open system.

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preliminary photosynthesis measurements of selected wheat cultivars prior to inoculation

Preliminary photosynthesis measurements of selected wheat cultivars prior to inoculation

Dr. Ferenc HorváthDr. Barnabás Wodala

an open system
An opensystem

In an open system, photosynthesis and transpiration are computed

from the differences in CO2 and H2O between in-chamber conditions and pre-chamber conditions.

slide8

IRGA (InfraRed Gas Analizator)

CO2measurement: 4,26 μm

H2O measurement: 2,59 μm

Infraredlightsource (widespectrum)

Gasinlet

Gasoutlet

Filter

Detector

Measuringbox

Signalprocessing

Indicator

incoming concentrations must be stable
Incoming concentrations must be stable

breath, for example, is typically 30000 μmol CO2 (mol air)-1

Ambient air contains360μmol CO2 (mol air)-1

slide12

Light

Reflectedlight

Mehlerreaction

ETR

PSI

PSII

ΦPSII

Photosynthesis

Transmittedlight

Photorespiration

Chlorophyll a fluorescence

Heatdissipation

preliminary measurements
Settinguptheinstrument (Licor 6400, an openpathgasexchangesystem)

4 selectedwheatgenotypes (1 weekbeforetheinfection)

PARout: sunny, dependsonangle, 600 – 1800 µmolphoton m-2 s-1

PARin: fixed, 1000 µmolphoton m-2s-1 (10% blue, 90% red)

Tair: 27-29 °C

Tleaf: 25-27 °C

preliminary measurements