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BIO 240 HISTOLOGY REVIEW Bone Tissue. Dr. Tim Ballard Department of Biology and Marine Biology. Compact bone. Human bone – ground – cross section -- India ink – 4x objective.

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BIO 240 HISTOLOGY REVIEWBone Tissue

Dr. Tim Ballard

Department of Biology and Marine Biology

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Compact bone

Human bone – ground – cross section -- India ink – 4x objective

This is a section of dried and long dead ground compact bone. The living cells are gone, leaving behind spaces that can be back-filled with India ink.

The arrowheads indicate osteons or Haversian systems. An osteon is that bony tissue surrounding and a part of a single Haversian canal.

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Compact bone

Human bone – ground – cross section -- India ink – 10x objective

osteon

interstitial lamellae

Interstitial lamellae are layers of old bone tissue from an osteon that is bring destroyed by remodeling.

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Compact bone

Human bone – ground – cross section -- India ink – 20x objective

lamellae

osteocytic lacuna

Haversian canal

interstitial lamellae

interstitial lamellae

Each osteon runs more-or-less parallel to the long axis of the bone. If you see a canal that is perpendicular, it is a Volkmann’s canal.

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Compact bone

Human bone – ground – cross section -- India ink – 40x objective

lamellae

canaliculi

Haversian canal

osteocytic lacuna

Canaliculi are tiny canals passing through the bone matrix that connect one osteocyte to another, ultimately connecting them all to the Haversian canal.