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Hormone receptor @ grade 2. double pos. % of patients. ER+ PR+ HER2-. Methods. triple pos. ER+ PR+ HER2+. Estrogen positive. Estrogen negative. Metabolic phenotype of HER2 +/- grade 2 tumors. Results. 241.1024 M-15. %. 147.0657. 257.1409 M+H. then: CI_GCT.

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Methods

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  1. Hormone receptor @ grade 2 double pos. % of patients ER+ PR+ HER2- Methods triple pos. ER+ PR+ HER2+ Estrogen positive Estrogen negative

  2. Metabolic phenotype of HER2 +/- grade 2 tumors Results

  3. 241.1024 M-15 % 147.0657 257.1409 M+H then: CI_GCT 189.9976 275.1654 Madd 0 100 200 m/z C5H6N2O2 PubChemChemSpiderCSLS DB Can we identify unknowns? 5-methyluracil3-methyluracil1-methyluracil… Methods

  4. Conclusion • Metabolomics is mature for discovery research. ~15% CV. More than one platform is needed due to chemical diversity.Databases are essential. • Once new biomarker panels are established, clinical validation could use less sophisticated techniques. • Breast cancer tumors vastly differ metabolically from normal to grade 1-3 • Triple negative tumors (ER-,PR-,HER2-) were mostly grade 3. • Metabolomics yields novel hypotheses on clinically relevant questions: Triple negative tumors have dysregulation of several metabolic modules: amino acids, nucleotide, membrane lipids and energy metabolism, plus salicylate and oligosaccharide metabolism. • Note: steady state levels may not change (pyruvate, free fatty acids, glutamine) although metabolic fluxes may be higher (as indicated in endpoint accumulations or products). More mechanistic insights by stable isotope studies. Conclusion

  5. Thanks to fiehnlab.ucdavis.edu ! Thanks to MetaCancer collaborators , PI Prof Carsten Denkert, Charite Berlin, Germany European Union FP7 Health-2007-2.1.4.1 project 200327 NIH R01 ES013932, R01 DK078328, RC2 GM092729, R01 HD058556

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