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# Reaksi Enzim PowerPoint PPT Presentation

Reaksi Enzim. Reaksi Enzim. Reaksi Enzim. Kinetika Enzim. Leonor Michaelis & Maud Menten. Persamaan Michaelis-Menten :. V 0  Laju / kecepatan awal enzim Vmaks  Laju / Kecepatan maksimum enzim. Km  Konstanta Mechaelis-Menten ( konsentrasi substrat pada saat

Reaksi Enzim

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Reaksi Enzim

### KinetikaEnzim

Leonor Michaelis & Maud Menten

PersamaanMichaelis-Menten:

V0 Laju/kecepatanawalenzim

Vmaks  Laju/Kecepatanmaksimumenzim

kecepatansetengahdariVmaks)

[S]  KonsentrasiSubstrat

### KinetikaEnzim

• Bila [S]  Km , maka V berbanding lurusdengan (S)

• Bila [S] = Km , maka V = 1/2 Vmax

• Bila [S]  Km , maka V = Vmax

### Penentuan Km danVmax

Plot double reciprocalatau

plot Lineweaver-Burk

• Plot 1/[S] vs 1/Vo

• Persamaan L-B (garislurus/linier)

• X-intercept = -1/Km

• Y-intercept = 1/Vmax

Penentuan Km danVmax

V max

Km

Km ~ 1.3 mM

Vmax ~ 0.25

Penentuan Km danVmax

-1/Km = -0.8

Km = 1.23 mM

1/Vmax = 4.0

Vmax = 0.25

### Km ???

• Km = [S] pada ½ Vmax

• Km affinitasenzimdengansubstrat

• 3. Km dapatdigunakanuntukmengevaluasispesifisitasenzimuntukberikatandengansubstrat

• Nilai Km kecilartinyaaffinitasenzim-substrattinggi; sebaliknya Km tinggiaffinitasenzim-substratrendah

GlucoseKm = 8 X 10-6

AlloseKm = 8 X 10-3

MannoseKm = 5 X 10-6

HexoseKinase

Glucose + ATP <-> Glucose-6-P + ADP

InhibisiEnzim

Inhibitor reveribel

E + S <-> ES -> E + P

E + I <-> EI

Ki = [E][I]/[EI]

• Competitive

• Non-competitive

### InhibisiEnzim

• Inhibitor mempunyaistruktur yang miripdengansubstrat

• Efek inhibitor dapatdihilangkandenganpenambahansubstrat

• Kompetitif:

Inhibisi Enzim

• Dapatmengikatenzimbebas (E) maupunkompleksenzim-substrat

Non-Kompetitif

Plot Lineweaver-Burk

Kompetitif

Non-Kompetitif

-COOH

H2N-

-SONH2

H2N-

### Aplikasi Inhibitor

Para-aminobenzoic acid (PABA)

Bakterimemerlukan PABA

Untukbiosintesisasamfolat,

sehinggabakteridapattumbuh

Domagk (1939)

• sehinggabakteritidakdapattumbuh

• Sebagai inhibitor enzim

• Inhibitor kompetitif

Sulfanilamide

Sulfa drug (anti-inflammation)

Thank You!

Quiz