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Principles of PCR

Principles of PCR. MUPGRET Workshop June 18, 2004. PCR. Polymerase Chain Reaction http://www.dnai.org/b/index.html http://palou.uib.es/roolpi/PCR/. Basic PCR.

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Principles of PCR

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  1. Principles of PCR MUPGRET Workshop June 18, 2004

  2. PCR • Polymerase Chain Reaction • http://www.dnai.org/b/index.html • http://palou.uib.es/roolpi/PCR/

  3. Basic PCR • Initially reagents in excess, template and product don’t renature and outcompete primer binding, so amplification occurs at an exponential rate. • Timepoint when exponential turns to linear rate is different depending on the initial concentration of the target molecule.

  4. Basic PCR • To properly quantify one product relative to another you need to be in the exponential phase of amplification for both and know when the end point occurs.

  5. Troubleshooting PCR • http://www.promega.com/guides/pcr_guide/070_22/promega.html • http://info.med.yale.edu/genetics/ward/tavi/Trblesht.html • http://palou.uib.es/roolpi/PCR/

  6. Electrophoresis • A way to separate DNA molecules. • http://gslc.genetics.utah.edu/units/biotech/gel/

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