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PCR. PCR AMPLIFICATION. PCR APPLICATIONS. MOLECULAR BIOLOGY ENVIRONMENTAL SCIENCE FORENSIC/MEDICAL SCIENCE BIOTECHNOLOGY GENETICS GENE CLONING MICROBIOLOGY. DNA STRUCTURE. SUGAR-PHOSPHATE BACKBONE A=ADENINE T=THYMINE C=CYTOSINE G=GUANINE. PCR REQUIREMENTS. DNA PRIMERS

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Pcr applications l.jpg
PCR APPLICATIONS

  • MOLECULAR BIOLOGY

  • ENVIRONMENTAL SCIENCE

  • FORENSIC/MEDICAL SCIENCE

  • BIOTECHNOLOGY

  • GENETICS

  • GENE CLONING

  • MICROBIOLOGY


Dna structure l.jpg
DNA STRUCTURE

  • SUGAR-PHOSPHATE BACKBONE

  • A=ADENINE

  • T=THYMINE

  • C=CYTOSINE

  • G=GUANINE


Pcr requirements l.jpg
PCR REQUIREMENTS

  • DNA

  • PRIMERS

  • dATP, dTTP, dCTP, dGTP

  • POLYMERASES


Rapd pcr l.jpg
RAPD PCR

  • RANDOM

  • AMPLIFIED

  • POLYMORPHIC

  • DNA


Rapd applications l.jpg
RAPD APPLICATIONS

  • DNA FINGERPRINTING

  • ANALYSIS OF ANCIENT DNA FROM FOSSILS

  • MAPPING THE HUMAN GENOME

  • FINGERPRINTING UNKNOWN MICROORGANISMS


Rapd primers l.jpg
RAPD PRIMERS

  • 10 NUCLEOTIDES LONG

  • ARBITRARY SEQUENCE

  • BINDS AT MULTIPLE SEQUENCES ALONG DNA

  • NON-SPECIFIC BINDING

  • ACCURACY REQUIRES AT LEAST 10 PRIMERS


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