Unveiling Cellular Respiration: Key Processes and Significance in Energy Release

1. Chapter 9 Cellular Respiration: Harvesting Chemical Energy

2. Is this a process of turning food into energy?

3. Respiration - Equation C6H12O6 + 6 O2 6 CO2 + 6 H2O and energy The energy is released from the chemical bonds in the complex organic molecules.

4. Respiration - Preview • The process of releasing Energy from food. • Food - Stored Energy in chemical bonds. • ATP - Useable Energy for cell work.

6. Focus of Chapter 1. Purpose - what is the reaction suppose to do? 2. Location - where is it at? 3. Requirements - what is needed to make it run? 4. Products - what does it produce?

7. Oxidation - definitions • Loss of electrons. • Loss of energy. • Loss of Hydrogens from Carbons.

8. Reduction - definitions • Gain of electrons. • Gain of energy. • Gain of Hydrogens to Carbons. Comment - be careful not to use “reduction” in lay terms.

9. Redox reactions • LEO the lion says GER!!!!!!

10. Redox reactions • Reactions are usually paired or linked together. • Look for these links as we study Respiration. • Many of the reactions will be done by phosphorylation

11. Phosphorylation • Adding a phosphate group to a molecule. • The phosphate group adds “energy” to the molecule for chemical reactions.

12. Phosphorylation

13. Cell Respiration - parts • Glycolysis • Oxidation of Pyruvate 3. Krebs Cycle 4. Oxidative Phosphorylation and the Electron Transport Chain

14. Glycolysis • Glyco- glucose. • -lysis: to split • Universal step in all Respiration types. • Likely to earliest type of cell energy processes.

15. Glycolysis • Function - To split glucose and produce NADH and ATP. • Location - Cytoplasm.

16. Electron Carrier Compounds • Molecules that transport or shuttle electrons within the cell. • Exist it two forms: • Oxidized (ox) • Reduced (red)

17. NAD • Nicotinamide Adenine Dinucleotide NAD+ + 2 e- NADH NAD+ = oxidized form NADH = reduced form

19. Glycolysis -Requirements • Glucose • 2 ATP • 4 ADP • 2 NAD+

20. Glycolysis - Products • 2 Pyruvic Acids (a 3C acid) • 2 ADP • 4 ATP • 2 NADH

21. Net Result • 2 ATP per glucose • 2 NADH

23. Energy Investment Phase

24. Energy Harvest Phase

25. Oxidation of Pyruvate (formation of Acetyl CoA)

26. Krebs Cycle • Also called: • Citric Acid Cycle • Tricarboxylic Acid Cycle

27. Krebs Cycle • Function: Complete oxidation of original glucose • Produce NADH and FADH2 • Location: Mitochondria matrix

28. Krebs Cycle -Requirements • Acetyl CoA (2C compound) • 3 NAD+ • 1 ADP • 1 FAD • Double this list for each glucose.

29. Krebs Cycle - Products • 2 CO2 • 3 NADH • 1 ATP • 1 FADH2 • Double this list for each glucose.

32. Krebs Cycle • Produces most of the cell's energy in the form of NADH and FADH2 • Does NOT require O2

33. Comment • The ATPs produced directly in Krebs Cycle and in Glycolysis are by: • Substrate-level phosphorylation • The Pi group is transferred from a substrate to ADP.

34. Electron Transport ChainOxidative Phosphorylation • ETC or Electron Transport System (ETS). • A collection of proteins that are structurally linked into units.

35. ETC • Uses sets of Cytochromes, Fe containing proteins to pass electrons. • The Cytochromes alternate between RED and OX forms and pass electrons down to O2

37. ETC and Oxidative Phosphorylation • Function: Convert NADH and FADH2 into ATP. • Location: Mitochondria cristae.

38. ETC - Requirements • NADH or FADH2 • ADP • O2

39. ETC - Products • NAD+ and FAD • ATP • H2O

40. Movie

41. ETC - ATP Yields • Each NADH -- 3 ATP • Each FADH2 -- 2 ATP

42. Chemiosmotic Hypothesis • ETC energy is used to move H+ (protons) across the cristae membrane. • ATP is generated as the H+ diffuse back into the matrix.

44. ATP Synthase • Uses the flow of H+ to make ATP. • Works like an ion pump in reverse, or like a waterwheel under the flow of H+ “water”.

46. Alcoholic Fermentation • Done by yeast, a kind of fungus.

48. Alcoholic Fermentation • Uses only Glycolysis. • An incomplete oxidation - energy is still left in the products (alcohol). • Does NOT require O2 • Produces ATP when O2 is not available.

50. Lactic Acid Fermentation • Uses only Glycolysis. • An incomplete oxidation - energy is still left in the products (lactic acid). • Does NOT require O2 • Produces ATP when O2 is not available.