PTRM Student Presentation Dan Wang 10-9-2008. Gentamicin treatment promotes dystrophin expression in mdx myotubes. Immunohistochemistry of myotubes from primary cell culture from muscles
There is a window of gentamicin dosage in which misreading can lead to the restoration of full-length dystrophin synthesis.
Eccentric contraction protocol
mdx EDL (extensor digitorum longus)muscles
Gi200: injection, 200% dose equivalent
GP: infusion pumps
D: DHB treatment
Procion orange (fluorescent dye) can enter muscle cells only through membrane disruptions.
Mice were subjected to eccentric contractions in the presence of Procion orange.
Tibialis anterior (TA) muscle cross-sections
mdx muscle (untreated)
mdx muscle (Gi200)
mdx muscle (Gi50F)
mdx muscle (GP200)
Immunoblot analysis of TA muscles for dystrophin and -sarcoglycan.
HEK293 cells stably transfected with LUC-190 gene
HeLa cell-free extract containing synthetic LUC-190 mRNA
Differences in transcript levels?
a. Force per cross-sectional area of treatment
b. Eccentric contraction assay
c. Serum creatine kinase changes
d. Western blot of quadriceps and TA muscles