1 / 6

Connecting Minimal Synthetic Biology with Millibiology

Connecting Minimal Synthetic Biology with Millibiology. 16-Oct-2008 DARPA Cambridge, MA. Classification of replication/reproduction How close to atomic inputs ? (e.g. autotrophs use CO2, N2, etc.) Non-translational, non-universal construction replication:

xander
Download Presentation

Connecting Minimal Synthetic Biology with Millibiology

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Connecting Minimal Synthetic Biology with Millibiology 16-Oct-2008 DARPA Cambridge, MA • Classification of replication/reproduction • How close to atomic inputs ? • (e.g. autotrophs use CO2, N2, etc.) • Non-translational, non-universal construction replication: • Schulman & Winfree. ECAL 2005. Self-Replication and Evolution of DNA • crystals • Sievers D, von Kiedrowski G.Nature. 1994 Self-replication of complementary • nucleotide-based oligomers.

  2. 3 Exponential technologies“law of accelerating returns” Computation & Communication tRNA urea B12 Synthetic chemistry telegraph Analytic tRNA Shendure et al. 2004 Nature Reviews of Genetics. Kurzweil 2002; Moore 1965 (24 month doubling til 2020)

  3. $500 per 15Mbp NextGen Synthesis: off chip 8K Xeotron Photo-Generated Acid 12K Combimatrix Electrolytic 120K Roche, Febit Photolabile 5'protection 244K Agilent Ink-jet standard reagents Application to shRNA & exon capture libraries Tian et al. 2004 Nature Elledge, Hannon, et al.

  4. Not minimal genomesHigh speed & accuracy • Mycoplasma 500 RNA & protein genes • Mitochondria 1500 genes • Mike Jewett E.coli in vitro151 genes • Ease of synthesis • More radical designs • Existing applications • -Roche Rapid Translation System (RTS) • -Ambion ActivePro In Vitro Translation Kit • -Novagen, Promega, Invitrogen, Qiagen • Stratagene, Paragon, Amersham

  5. Not minimal:High speed & accuracy requires a few extra genes(E.coli 20 min. doubling) Reconstituted ribosomes:Jewett & Church 113 kbp DNA 151 genes Pure translation: Forster & Church MSB ’05 GenomeRes.’06 Shimizu, Ueda ’01

  6. New genetic code: viral-resistance, novel amino acidsno functional GMO DNA exchange PEG-pAcPhe-hGH (Ambrx) high serum stability 314 TAG to TAA changes 4 Charalel Church Isaacs Sun Wang Carr Jacobson Kong Sterling 1 3 2

More Related