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1. Atomic Absorption Spectroscopy. By H isham E Abdellatef. Advanced Flame and Graphite Furnace Atomic Absorption  Spectroscopy Course Instructor:  prof. Dr.  Hisham Ezzat Abdellatef. Introduction. AAS is used to determine metal element concentration in a sample

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Atomic Absorption Spectroscopy


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    1. 1 Atomic Absorption Spectroscopy By Hisham E Abdellatef

    2. Advanced Flame and Graphite Furnace Atomic Absorption Spectroscopy Course Instructor: prof. Dr.  HishamEzzatAbdellatef

    3. Introduction • AAS is used to determine metal element concentration in a sample • Can analyze over 62 metal elements • Cold Vapor AA, Flame AA, and Graphite furnace AA • Standard addition and standard calibration technique can be used to compute for metal element concentration

    4. Technique – Flame Test

    5. Atomic Absorption Spectroscopy:An Aussie Invention • Developed by Alan Walsh in early 1950s.

    6. Introduction

    7. ATOMS Bohr’s shell model: SODIUM atom • Nucleus- protons (+ve) and neutrons (neutral). • Electrons- (-ve) charged particle. • Shells- consists of subshells. Electrons Nucleus Shells

    8. Shell, Subshells, Electrons • Electrons are arranged according to their energy levels. They are arrange in subshells, the subshells are arrange in shells and shells are arrange around the nucleus. Atom (around the nucleus) Shells !Note: Electrons that are near the nucleus has a lower energy level than the electrons that are much far away. However, they experience stronger attraction in the nucleus than those ones that are further away. Subshells Electrons

    9. ‘Exciting’ atoms • ‘ground’ state: is a status where the atom’s electrons are in their lowest possible energy level. (stable) • ‘excited’ state: another status where the atom’s electrons absorb enough energy to be promoted to a higher level. Therefore, they are not in their lowest energy level. (unstable)

    10. ‘Excited’ atom Since, an atom’s excited state is very unstable it rapidly ‘jump’ back down to its ground state. This ‘jump’ then causes the atom to release the energy it absorbed in the form of photons of light. Generally, atoms are in their ‘ground state’ but when an atom receives enough input of energy that their electrons requires to be promoted to a higher energy level. They will then turn to their ‘excited state’. !Note: Take note that the electron can go back down to its ‘original’ place in more than one jump. Ground state: sodium atom Excited state: sodium atom