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I nsertion of Fragments of DNA: G ene D isruption and Replacement

I nsertion of Fragments of DNA: G ene D isruption and Replacement. Gene transfer in murine system. Several different combinations of growth factors have been successfully used for supporting gene transfer into murine stem cells.

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I nsertion of Fragments of DNA: G ene D isruption and Replacement

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  1. Insertion of Fragments of DNA: Gene Disruption and Replacement

  2. Gene transfer in murine system • Several different combinations of growth factors have been successfully used for supporting gene transfer into murine stem cells. • Combination of interleukin 3 (IL-3), interleukin 6 (IL-6), and stem cell factor (SCF). Inclusion of recently discovered early acting growth factors such as flt-3 ligand and megakaryocyte growth and development factor (MGDF)/thrombopoietin (TPO) have augmented the level of genetically modified cells. • Treatment of animals with 5-fluorouracil before marrow harvest to stimulate cycling of primitive cells, • The co-culture of target cells directly on a layer of retroviral producer cells or other stromal support, • The use of high titer (greater than 105viral particles per ml) vector and • Co-localization of vector and target cells using fibronectin-coated dishes.

  3. The T cell membrane (shown as the purple, semicircle double line) allows entry of HIV (in pink) into the cell through multiple cell receptors anchored on the membrane, including CCR5.

  4. Use of Genetically Modified Stem Cells in Experimental Gene Therapies

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