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This study involves the analysis of posterior hypothalamus tissue in Sprague-Dawley rats using a gel with pH3-10NL range and Cy3/Cy5 labeling. Tissue samples were treated with a buffer containing 7M Urea/.2M Thiourea and NP40. Gel was made by Applied Biomics.
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Tissue: Posterior hypothalamus, Sprague-Dawley Rats Buffer: 7M Urea/ 2M Thiourea + NP40 Gel: pH3-10NL, 9-16%, 1.5mm, Cy3/Cy5 labeling made by Applied Biomics (http://www.appliedbiomics.com)