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##### Process of Genetic Epidemiology

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**Process of Genetic Epidemiology**Defining the Phenotype Migrant Studies Familial Aggregation Segregation Linkage Analysis Association Studies Cloning Fine Mapping Characterization**Familial Aggregation**• Does the phenotype tend to run in families?**Recurrence (‘Familial’) Risk Ratios**• Compares the probability a subject is affected given they have an affected family member to the population risk: lR = KR/K, where KR is the risk to relatives of type R K is the population risk lS = recurrence risk to siblings of probands versus the general population risk.**Recurrence Risk Ratios**lR = P(Y2 = 1 |Y1 = 1) / K P(Y2=1|Y1=1)P(Y1=1) = P(Y2=1, Y1=1) P(Y2=1|Y1=1) = P(Y2=1, Y1=1)/P(Y1=1) K = P(Y1=1) lR = P(Y2=1, Y1=1)/P(Y1=1)2**Estimating RRR**• With case-control data, calculate FRR as: Proportion of affected relatives of the cases (observed) / Proportion of affected relatives of controls (expected) (assumed to estimate K) • The higher the value of l, the stronger the genetic effect**Examples of s**• Alzheimer Disease 3-4 • Rheumatoid Arthritis 12 • Schizophrenia 13 • Type I Diabetes 15 • Multiple Sclerosis 20-30 • Neural Tube Defects 25-50 • Autism 75-150**s versus GRR**• How well does s estimate the genetic risk ratio? s = P(Y2=1|Y1=1) / P(Y2=1|Y1=0) ? P(Y2=1|D) / P(Y2=1|dd) = GRR Sibs disease not necessarily hereditary At risk individual may not have inherited D Sib unaffected doesn’t mean other sib doesn’t carry D**Heritability Analysis**• Evaluates the genetic contribution to a trait Y in terms of variance explained. • Y = Genetics + Environment • Var(Y) = overall variation in phenotype Y = Var(G) + Var(E) + 2Cov(G,E) • Broad sense heritability: H2 = Var (G) / Var (Y) where Var(G) = genetic part of variance = VA+VD (Additive + Domince)**Narrow Sense Heritability**• Proportion of phenotypic variance that is explained only by additive genetic effects: h2= VA/ Var (Y) A number of ways to estimate heritability. Commonly done with twin studies.**Twin Studies**• Compare the phenotype correlation or disease concordance rates of MZ (identical) and DZ (fraternal) twins. Twin 1**MZ Twins (Identical)**Twin 1 Both alleles are shared identical by descent (IBD) Twin 2**DZ Twins (Fraternal)**Twin 1 Twin 2: any of the four IBD can be 2, 1, or 0 2 1 1 0**DZ Twins (Fraternal)**Twin 1 Average sharing is 50% 100% 50% 50% 0%**IBD Sharing**# of alleles shared IBD 2 1 0 Pr(2) Pr(1) Pr(0) Prop IBD Relationship Self, MZ twins 1 0 0 1 Parent, Offspring 0 1 0 1/2 Full siblings 1/4 1/2 1/4 1/2 Gr-child, Gr-prt 0 1/4 3/4 1/4 First cousins 0 1/4 3/4 1/8 Proportion of alleles shared IBD = # alleles x Pr(# alleles) / 2**Twin Studies**• Assume MZ twins share all genes & envt., DZ share ½ genes & all envt. • Correlation among twins: rmz= VA+ VE rdz= ½ VA + VE where VE = common environment H2 = 2(rmz- rdz ) • Heritability ~ two times difference in correlation between MZ and DZ twins.**Example of Twin Study: PCa**• Twin registry (Sweden, Denmark, and Finland) • 7,231 MZ and 13,769 DZ Twins (male) Heritability: 0.42 (0.29-0.50) Non-shared Environment: 0.58 (0.50-0.67) Lichtenstein et al NEJM 2000 13;343:78-85. Limitations of heritability calculations?**Segregation Analysis**• Study families. • Estimate ‘mode of inheritance’ & what type of genetic variant might be causal. • Determine whether the disease appears to follow particular patterns across generations. • Estimate whether variants are rare or common, etc.**Segregation: Harry Potter’s Pedigree**Muggle Wizard / Witch Vernon Dursley Lily Evans James Potter Petunia Dursley Harry Potter Dudley Dursley**Segregation Analysis**• What is the best model of inheritance for observed families? • Dominant • Recessive • Additive • Disease allele frequency? • Magnitude of risk? • Fit formal genetic models to data on disease phenotypes of family members. • The parameters of the model are generally fitted finding the values that maximize the probability (likelihood) of the observed data. • This information is useful in parametric linkage analysis, which assumes a defined model of inheritance.