1 / 2

Genetic Regulation and Protein Localization by lacO in Transgenic Studies

Explore the role of lacO in transgenic models to regulate gene expression and visualize protein localization. Techniques include ChIP-qPCR and various molecular biology methods. Published works by Rosin et al. (2008), Jovtchev et al. (2008), Matzke et al. (2005), Kato and Lam (2001) are featured.

ulf
Download Presentation

Genetic Regulation and Protein Localization by lacO in Transgenic Studies

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. lacO (~2 kb) Pd35S-IAAH-ter ter-GUS-minP35S 1 kb LB RB Ds5 Ds3 CCP4.193 PNOS-NPTII-tNOS ter-LUC-P35S Rosin et al. (2008) Jovtchev et al. (2008) lacO (~2 kb) ter-GUS-minP35S Ds5 Ds3 CCT431 PNOS-NPTII-tNOS Rosin et al. (2008) Jovtchev et al. (2008) ter-LUC-P35S tE9-GVG-P35S LB 256 x lacO (9.3 kb) RB NPTIIbac 112, 125 P35S-dsRed-LacI-35Ster tNOS-NOS-PNOS Matzke et al. (2005) Jovtchev et al. (2008) PNOS-NPTII-NOSt PNOS-NPTII-NOSt 35Ster-LacI-GFP-P35S LB 256 x lacO (9.3 kb) RB NPTIIbac 26 tNOS-NOS-PNOS Matzke et al. (2005) Jovtchev et al. (2008) t3A-NLS-LacI-GFP-TATA-6xUAS tE9-GVG-P35S LB 256 x lacO (10.1 kb) RB tNOS-HPT-PNOS ChIP-qPCR EL702C Kato and Lam (2001) Pecinka et al. (2005) Jovtchev et al. Suppl. Figure 1a

  2. lacO 10 kb lacO 17 kb lacO ~2 kb lacO 9.3 kb Jovtchev et al. Suppl. Figure 1b

More Related