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This study investigates the effects of Dynasore on human macrophages (MΦ) in terms of cell viability and Wnt5a expression. Human MΦ were treated with Dynasore for 72 hours, and cell vitality was assessed using the MTT assay, showing no significant impact (n=4, mean±SD). Additionally, treatment with Dynasore alone for 24 hours did not alter the mRNA levels of Wnt5a as measured by qRT-PCR (n=10, mean±SD). These findings suggest that Dynasore does not affect macrophage cell viability or Wnt5a expression levels.
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Suppl. Figure 1 A B Suppl. Fig. 1: Dynasore does not affect MΦcell viability or cellular Wnt 5a expression. (A), Human Mɸ were treated for 72h with dynasore and cell vitality was measured by MTT assay (n=4, mean±SD) (B), Treatment with dynasore alone for 24h has no effect on the MɸWnt 5a mRNA level in qRT-PCR experiments (n=10, mean±SD).