Infect TIVE
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Infect TIVE with BCBL-1 virus. TIVE KSHV + day 7 Ad-orf50 day 4. SLK KSHV + Ad-orf50. TIVE KSHV + day 7. TIVE KSHV -. 4 days post Inf. LANA IFA. K8.1. Induce lytic cycle TPA 6 days. Purify virus from TIVE; infect fresh TIVE cells. LANA IFA 4 days post Inf. Repeat 3 times.

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Infect TIVE

with BCBL-1 virus

TIVE KSHV + day 7

Ad-orf50 day 4

SLK KSHV +

Ad-orf50

TIVE KSHV +

day 7

TIVE KSHV -

4 days post Inf.

LANA IFA

K8.1

Induce lytic cycle

TPA 6 days

Purify virus from

TIVE; infect fresh

TIVE cells

LANA IFA

4 days post Inf.

Repeat 3 times

Suppl. Figure 2

A

B

C


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