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Infectious Characterization of BCBL-1 Virus and KSHV Lytic Cycle Induction

This study explores the infectious behavior of BCBL-1 virus and KSHV in cultured cells. It examines the induction of the lytic cycle using Ad-orf50 at different time points (day 4 and day 7) and involves assessing infection in fresh TIVE cells. The methodology includes immunofluorescence analysis with LANA and IFA to monitor viral expression post-infection. The findings contribute to a deeper understanding of KSHV dynamics and viral replication processes.

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Infectious Characterization of BCBL-1 Virus and KSHV Lytic Cycle Induction

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  1. Infect TIVE with BCBL-1 virus TIVE KSHV + day 7 Ad-orf50 day 4 SLK KSHV + Ad-orf50 TIVE KSHV + day 7 TIVE KSHV - 4 days post Inf. LANA IFA K8.1 Induce lytic cycle TPA 6 days Purify virus from TIVE; infect fresh TIVE cells LANA IFA 4 days post Inf. Repeat 3 times Suppl. Figure 2 A B C

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