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Chapter 11. Transposition and Site-Specific Recombination. 29 September and 1 October, 2004. Overview. Conservative Site-Specific Recombination (CSSR) may involve insertion, deletion, or inversion of DNA sequences.

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Chapter 11

Chapter 11

Transposition and Site-Specific Recombination

29 September and 1 October, 2004


Overview
Overview

  • Conservative Site-Specific Recombination (CSSR) may involve insertion, deletion, or inversion of DNA sequences.

  • Site-specific recombinases have a mechanism that includes a DNA-protein covalent intermediate.

  • CSSR may be regulated by the presence or absence of accessory proteins.

  • Resolvases are CSSR recombinases that disentangle circular chromosomes.

  • Transposons move using recombination pathways.

  • Transposons may be autonomous or nonautonomous.

  • DNA transposons and viral-like retrotransposons move via a cut/paste mechanism.

  • Retrotransposon movement involves reverse transcription.

  • Some transposons regulate copy number or control target site selection through the use of proteins or antisense RNA.

  • V(D)J recombination uses regulated, specific recombination to generate immune diversity.












L int and ihf stabilize bent dna
lInt and IHF stabilize bent DNA.

















Line poly a retrotransposon movement

LINE poly-A Retrotransposon Movement the same protein superfamily.








Antibody

Antibody immunity.





Title

Title immunity.