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Summary of Solute Transport. AP Biology. Solute Transport – three types. Three types Diffusion Facilitated Diffusion Active Transport. Comparison of Transport Mechanisms. Problem.
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Summary of Solute Transport AP Biology
Solute Transport – three types • Three types • Diffusion • Facilitated Diffusion • Active Transport
Problem • Aquaporins are channel proteins that increase the membrane permeability to water. Water permeability was tested by injecting oocytes(which normally do not have aquaporins) with +/- mRNA and incubating in a hypotonic solution. After time X, oocytes were not longer visible • Why did the cells with aquaporin mRNA increase in volume? • What happened at time X? • How would you estimate the relative rates (increase volume per min) of swelling?
Think-Pair-Share An experiment is designed to study the uptake of sucrose by plant cells. Cells are immersed in a sucrose solution and the pH of the solution is monitored. Samples of cells are taken at different time intervals and their sucrose concentration is measured. If the surrounding solution has a pH of 5.5, sucrose uptake occurs but not if the surrounding solution is pH of 8. Why? 3 PROPERTY OF PIMA COUNTY JTED, 2010
Large macromolecules • Cannot move across the membrane by transport proteins- too large • Must use another mechanism • Exocytosis-macromolecules exiting cell • Endocytosis – macromolecules entering cell • Pinocytosis –move in fluids • Phagocytosis – move in particulate matter • Receptor mediated – concentrate specific solutes
Osmosis Lab • Read procedures A and B • Purpose of Procedure A? • Purpose of Procedure B?