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Cloning. Course: Bioinformatics Presented by: Adil Hussain - 0612120. Cloning. Creating an exact organism Naturally Artificially Process Somatic Cell Nuclear Transfer Artificial Embryo Twinning The Roslin Technique. DNA in a Cell. Somatic Cell Nuclear Transfer. Somatic Cell

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cloning

Cloning

Course:

Bioinformatics

Presented by:

Adil Hussain - 0612120

cloning1
Cloning
  • Creating an exact organism
    • Naturally
    • Artificially
  • Process
    • Somatic Cell Nuclear Transfer
    • Artificial Embryo Twinning
    • The Roslin Technique
somatic cell nuclear transfer
Somatic Cell Nuclear Transfer
  • Somatic Cell
    • Any cell of the body except

sperm or egg

  • Nucleus
    • Brain of the cell

PROCESS

artificial embryo twinning
Artificial Embryo Twinning
  • Simple cloning process
    • Copies the natural process
  • Naturally identical twins
  • Artificial twins

PROCESS

the roslin technique
The Roslin Technique
  • Roslin Institute
  • Variation of the Somatic Cell Nuclear Transfer
  • PROCESS
    • Somatic cells (nuclei intact) to grow & divide
    • Inactive state
    • Fused with egg cell (without nucleus)
    • Grow into embryo
    • Implanted in surrogate mother
cloning for cs students
Cloning for CS students
  • Complicated procedure
    • Problems
      • Multiplication of cells
      • Embryos not developing
      • Abnormalities in embryos
  • DNA controls cell transformation
    • 30,000 DNA in a single cell
dolly the cloned sheep
Dolly (the cloned sheep)
  • 5th July 1996 – 14th February 2003
  • First mammal to be created from an adult somatic cell
  • 1/277 attempts made Dolly
cloning in pakistan
Cloning in Pakistan
  • Pakistan is an agricultural country
  • Biotechnology to improve crops
  • SUGARCANE – 5th largest producer
    • 458 mounds per acre
    • 800-1000 mounds per acre
    • Diseases reduced production 10% – 77%
cloning in pakistan1
Cloning in Pakistan
  • AGRIBIOTECH
  • Plants are vigorous, healthy, disease free and of a premium quality.
  • Yield increases from 50% – 100%
  • Sugar recovery increase by 1 - 2 %
  • Reduces the seed requirement to one third
  • Reduce lodging which will also decrease the infestation of insects and pests.
  • Lower production cost due to high sugar and cane yield per unit area.
  • Vigorous growth and development results greater disease resistance.
  • Availability of high quality vigorous and disease free seed at affordable price round the year.
  • Every bud sprouts at least thrice
role of bioinformatics
Role of Bioinformatics
  • In Cloning
    • Ensuring integrity
    • Matching DNA
    • Extent of mutations
conclusion
Conclusion
  • Successful cloning is very difficult
    • Very low success rate
  • Stem cell research
    • Diseases (gene therapy)
    • Abnormalities
  • Gene Therapy
    • Gene screening to identify defective gene
    • Normal gene to correct it
    • Organ Transplant
  • Human reproductive cloning
questions
Questions?

Thank You