BIOL2450 APPLIED MICROBIOLOGY

1. BIOL2450 APPLIED MICROBIOLOGY Course Context: Allied health course for nursing students (non-traditional, diverse, low-income, women, and EFL). Four credit course with lab.

2. Learning Activities • Assigned textbook readings with list of terms to know and worksheets/crossword activity (pre-class) • Case Studies • Part 1: Extrapolate key information from case studies • Part 2: Conduct research and information gathering to analyze case studies • Part 3: Assemble information in case studies and from research to determine the etiological agent • Part 4: Construct a paper with correct citations and defend choice of microbe • Part 5: Peer review case study 1 with comments using grading rubric • Written assignment that process information gathering and decision making to solve case study. Flow Chart. (Assigned in class with examples and students conduct outside of class time) • Peer review classmate case study one (Demonstrated with guidelines in class and students are placed into groups of 4 on D2L and given 4 blinded case study to assess as a group)

3. Formative Assessments Matching: Match the following terms to its definition. __ pathology a) development of disease __ etiology b) results in both organisms benefiting __ pathogenesis c) study of disease __ parasitism d) study of cause of disease __ commensual e) results in one benefiting and the other being harmed __ mutualism f) results in one benefiting and the other is unaffected • Online quiz Multiple Choice: AIDS is one of the top three infectious disease worldwide. What would AIDS be classified as? Endemic Epidemic Pandemic Sporadic (2) Draft of Case Study 1 After student groups have reviewed case study one, I grade the quality of review as well as add my own comments. Denote (+) useful comments and (-) not relevant. Students will have a chance to correct case study one and apply those corrections to the remaining three case studies

4. Summative Assessments • Exam (2) Case Study 1, 2, 3, and 4 (Rubic) Fill-in-the-blank: The majority of normal flora in humans are considered to be ____ (type of symbiosis). Short Answer: A 27-year old woman was seen at the clinic for painful urination. She was diagnosed with a UTI caused by endogenous E. coli. Explain how and why this may have occurred.

5. Timeline Unit One: Microbial structure and function, metabolism, growth, and genetics (Fall 2012) Unit Two: Eukaryotic Microorganisms (Fungus, Protozoa, and Viruses) (Winter 2013) Unit Three: Symbiosis, Principles, and Pathogenicity(Summer 2012) Unit Four: Immunity and Antimicrobial Agents (Summer 2013) Unit Five: Microbial Diseases (Fall 2013)

7. Case study Assignment • DUE DATE: July 27 • Case Study Guidelines: • Case studies must be typed with Times New Roman, 1” margins, and double-spaced. • Include a cover sheet with your name and date. • This is a writing assignment; answers must be written using complete sentences, correct grammar, and spelling. You will have to say clearly how and why you came to this conclusion. If you are not sure about what the answer should be, you should explain why you couldn’t come to a conclusion • Write the names of the microorganisms in correct scientific notation. The full name of a microorganism must be used once before the abbreviated version can be used. • There is a min. requirement of 1 page and max. of 3 pages per case study. • Please keep in mind that the genus name of an organism is not the same as the full scientific name of an organism. The name of the disease/infection is often different from the name of the microorganism. • Each student must have distinctly separate answers (no paraphrasing!). Students that have similar typed answers will receive no credit and will not be able to resubmit case study. • Use proper citation (in text and reference page) and be consistent with your citation method • Case studies need to be submitted in paper by July 27 at the beginning of class (if you are late to class, your case studies will be considered late) – Students may submit case studies in advance by email/paper. • For each case study, answer the following questions: • 1. What is the etiological agent? Describe the morphology (shape, arrangement, gram reaction) and cultural properties (O2 requirement, biochemical requirements, motility, etc…) of this etiological agent. • 2. Explain the spread of infection (reservoir & transmission) and pathogenicity (3 virulent factors) of this agent relevant to this case study. • 3. Describe the diagnoses and treatment (name of Rx, what it targets, why does it work against this microbe) for this agent relevant to this case study. Case #1 Description: An 18-year-old male is admitted to the hospital with massive tissue destruction along her right leg. The skin is a violet color and large fluid-filled blisters are present. The patient has a fever, a rapid heart rate, low blood pressure, and seems confused. Once the area appeared to have become infected, the infection spread very rapidly. Lab diagnosis:

9. FLOW CHART SICK PT. • GATHER INFORMATION • Patient History: Location/Region, Mode of transmission, and Susceptibility. • Signs & Symptoms: Pathogenicity/Virulence factor • Lab Diagnosis: Gram stain (Shape, Arrangment, and Cell wall type), Biochemical tests (Growth requirements & Metabolism), and so on • **Identify possible suspects ** • CONDUCT RESEARCH • What information is CRITICAL? • Patient History • Signs & Symptoms • Lab Diagnosis ASSEMBLE & CONSTRUCT