Microbiology. Class Three Spring and Fall Semester Culture Characteristics. Day 3. Exp 2B, Isolation of pure cultures form SPD, Streak Plate Dilution, Technique. Procedure: page 15 & page 19 Exp 3, Culture Characteristics Discussion Procedure: page 23 Charts Case Study.
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Spring and Fall Semester
Quiz next Week
ML/SM mixture – circle yellow and pink cultures
Exp 2B, Isolation of pure cultures form SPD, Streak Plate Dilution, Technique.
Procedure: page 15 & page 19
Culture per table:
PA, EC,EA , PV,
SA, SS, BS, SE,
Pick, using aseptic technique and a needle, a unique colony and transfer to a labeled slant.
ML > yellow
BS > white
SM > red
Inoculate each culture into a labeled media
Prep for Incubation @ Room Temp
Prep for Incubation @ 37C
Exp 2A, Isolation of Pure Cultures
SM, Serratia marcescens,red
ML, Micrococcus luteus,yellow
BS, Bacillus subtilis, white
Materials: mixed cultures: SM/ML & BS/SM , one per table, 4 TSA Plates per person
Eight Organisms for Study/Table