microbiology l.
Download
Skip this Video
Loading SlideShow in 5 Seconds..
Microbiology PowerPoint Presentation
Download Presentation
Microbiology

Loading in 2 Seconds...

play fullscreen
1 / 20

Microbiology - PowerPoint PPT Presentation


  • 106 Views
  • Uploaded on

Microbiology. Class Three Spring and Fall Semester Culture Characteristics. Day 3. Exp 2B, Isolation of pure cultures form SPD, Streak Plate Dilution, Technique. Procedure: page 15 & page 19 Exp 3, Culture Characteristics Discussion Procedure: page 23 Charts Case Study.

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about 'Microbiology' - velvet


An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
microbiology

Microbiology

Class Three

Spring and Fall Semester

Culture Characteristics

day 3
Day 3
  • Exp 2B, Isolation of pure cultures form SPD, Streak Plate Dilution, Technique.
  • Procedure: page 15 & page 19
  • Exp 3, Culture Characteristics
  • Discussion
  • Procedure: page 23
  • Charts
  • Case Study
  • Review culture:
  • (Exp 1, 2A)
  • update Journals

Quiz next Week

class schedule part one
Class schedule( Part One)
  • Check results from class 3.
  • Record results in templates.
  • Organisms studied – class 3
  • S. marcescens
  • B. subtilis
  • M. luteus
culture characteristics
Culture characteristics
  • Use supplement and handout to observe the growth of the four organisms in the slant, deep, broth, and on the plate.
  • Do the organisms look like one of the examples on your sheet?
  • Try to record their appearance on your templates
culture observations on the agar plate
Culture observations on the agar plate
  • Color production( chromogenesis). An example of this is the pink color of Serratia
  • Growth pattern and characteristics
  • Amount of growth( scant or heavy)
broth culture refer to supplement
Broth culture( refer to supplement)
  • Cloudy
  • Turbid( Flocculent)
  • Sediment formation
  • Pellicle formation
slants
Slants
  • Is there growth in the bottom ?
  • Is there growth on the slant itself
  • What are the growth characteristics on the slant?
  • Key words

Aerobic

Anaerobic

Facultative

part one completion of class 3 work
Part One – Completion of class 3 work
  • When you have finished observing all of your cultures
  • Place all tubes in rack in hazardous waste
  • Place all plates in cans in hazardous waste
  • Wipe down desk top
ex 2 b isolation of pure culture
Ex# -2/B -Isolation of Pure culture
  • Observe your dilution streak of your mixed culture
  • On the bottom of your Petri dish circle colonies of two organisms
  • Example

ML/SM mixture – circle yellow and pink cultures

  • With your inoculating loop lift cells from circled colonies and streak on new plate or inoculate a slant per detailed instructions in class
slide15

Day 4

  • Exp 3, Culture Characteristics
  • Discussion
  • Procedure: page 23

Exp 2B, Isolation of pure cultures form SPD, Streak Plate Dilution, Technique.

Procedure: page 15 & page 19

Culture per table:

PA, EC,EA , PV,

SA, SS, BS, SE,

Pick, using aseptic technique and a needle, a unique colony and transfer to a labeled slant.

ML > yellow

BS > white

SM > red

Inoculate each culture into a labeled media

  • TSA plate, SPD
  • TSA slant, surface
  • TSA Broth

Per table

Prep for Incubation @ Room Temp

Prep for Incubation @ 37C

slide16

cultures

cultures

SM/ ML

SM/ ML

BS/SM

SP

SPD

review

Exp 2A, Isolation of Pure Cultures

  • Streak Plate Dilution Technique , SPD
  • Spread Plate Technique

SM, Serratia marcescens,red

ML, Micrococcus luteus,yellow

BS, Bacillus subtilis, white

BS/SM

22C/24hr

SPD,SM/ML RF

SPD,BS/SM RF

SM/ML RF

BS/SM RF

Materials: mixed cultures: SM/ML & BS/SM , one per table, 4 TSA Plates per person

new work supply table experiment 3
New work( supply table)Experiment #3

Eight Organisms for Study/Table

  • 8 Plates
  • 8 Deeps( if available)
  • 8 Slants
  • 8 Broths
key organisms for study
Key Organisms for study
  • Gram negative organisms
  • PA - Pseudomonas aeruginosa
  • PV- Proteus vulgaris
  • EC- Escherichia coli
  • EA- Enterobacter aerogenes
  • Gram Positive Organisms
  • BS - Bacillus subtilis
  • SA - Staphylococcus aureus
  • SE - Staphylococcus epidermidis
  • SS- Streptococcus salivarius
preparation
Preparation
  • Label all tubes and plates carefully
  • Assign each member of the group 2 organisms
  • Transfer the organisms to the culture media using aseptic techniques used in weeks one and two
review for quiz one
Review for Quiz One
  • Aseptic techniques
  • Culture transfer techniques
  • Media
  • Safety precautions
  • Microscope identification