Eukaryotic Gene Regulation April 23, 2014 Richard D. Howells, PhD MSB E-643 firstname.lastname@example.org. Objectives. During this presentation you may Catch another 40 winks, or Understand key concepts regarding regulation of gene expression in eukaryotes. Eukaryotic RNA Polymerases. 3.
Eukaryotic Gene Regulation April 23, 2014Richard D. Howells, PhDMSB Eemail@example.com
During this presentation you may
The poisonous mushroom, Amanita phalloides, contains -amanitin, a cyclic octapeptide that blocks elongation by RNA Pol II. It is lethal at 10 nM. The initial reaction to ingestion of the mushroom is gastrointestinal distress, and 48 h later the subject dies, usually from liver dysfunction.
From Transcriptional Control
Of Gene Expression, Molecular
Cell Biology, Lodish et al., eds.,
These and related lipid-soluble hormones bind to receptors located in the nucleus and cytoplasm. The hormone-receptor complex functions as a transcription factor in the nucleus.
The centrally located DNA binding domains each contain 2 copies of the
zinc finger motif. Ligand binding domains are C-terminal, and N-termini
often contain activation domains.
Repeat sequences are indicated by red arrows.
AD: activation domain; DBD: DNA binding domain; LBD: ligand binding domain
CRE: cAMP response element;
CREB: CRE binding protein; CBP: CREB binding protein
Mutation of CBP causes pleiotropic developmental abnormalities:Rubinstein-Taybi syndrome
post-translational modification of histone side chains
HAT: histoneacetyltransferase activity
HDAC: histonedeacetylase activity
The transcriptional activator Gcn5 has histoneacetyltransferase activity
The transcriptional repressor Rdp3 has histonedeacetylase activity
Example: Developmental-Temporal Regulation of Factor IX Transcription
Factor IX Transcribed Region
AR binding site
HNF4 binding site
Model for cleavage and polyadenylation of pre-mRNAs in mammalian cells
CPSF- cleavage and
polyadenylation specificity factor
CStF- cleavage stimulatory factor
CFI,CFII- cleavage factors
PAP- poly(A) polymerase
PABP II- poly(A) binding protein