Eukaryotic Gene Regulation April 23, 2014 Richard D. Howells, PhD MSB E-643 email@example.com. Objectives. During this presentation you may Catch another 40 winks, or Understand key concepts regarding regulation of gene expression in eukaryotes. Eukaryotic RNA Polymerases. 3.
Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.
Eukaryotic Gene Regulation April 23, 2014Richard D. Howells, PhDMSB Efirstname.lastname@example.org
During this presentation you may
The poisonous mushroom, Amanita phalloides, contains -amanitin, a cyclic octapeptide that blocks elongation by RNA Pol II. It is lethal at 10 nM. The initial reaction to ingestion of the mushroom is gastrointestinal distress, and 48 h later the subject dies, usually from liver dysfunction.
From Transcriptional Control
Of Gene Expression, Molecular
Cell Biology, Lodish et al., eds.,
These and related lipid-soluble hormones bind to receptors located in the nucleus and cytoplasm. The hormone-receptor complex functions as a transcription factor in the nucleus.
The centrally located DNA binding domains each contain 2 copies of the
zinc finger motif. Ligand binding domains are C-terminal, and N-termini
often contain activation domains.
Repeat sequences are indicated by red arrows.
AD: activation domain; DBD: DNA binding domain; LBD: ligand binding domain
CRE: cAMP response element;
CREB: CRE binding protein; CBP: CREB binding protein
Mutation of CBP causes pleiotropic developmental abnormalities:Rubinstein-Taybi syndrome
post-translational modification of histone side chains
HAT: histoneacetyltransferase activity
HDAC: histonedeacetylase activity
The transcriptional activator Gcn5 has histoneacetyltransferase activity
The transcriptional repressor Rdp3 has histonedeacetylase activity
Example: Developmental-Temporal Regulation of Factor IX Transcription
Factor IX Transcribed Region
AR binding site
HNF4 binding site
Model for cleavage and polyadenylation of pre-mRNAs in mammalian cells
CPSF- cleavage and
polyadenylation specificity factor
CStF- cleavage stimulatory factor
CFI,CFII- cleavage factors
PAP- poly(A) polymerase
PABP II- poly(A) binding protein