Wet Mount PT: 2007ASpecimen 1 • Evaluate micrographs 1-a, 1-b, 1-c and 1-d together as if different fields from the same patient • Identify cellular images labeled as: • 1 • 2 • 3 • Enter results on result sheet
Micrograph 1-a Low Power 3 2 1 2
Micrograph 1d High Power 2 3 2 3
Wet Mount PT: 2007A Specimen 2 • Evaluate micrographs 2-a, 2-b, and 2-c together as if different fields from the same patient • Identify cellular images labeled as: • 4 • 5 • 6 • Enter results on result sheet
Micrograph 2b – Low Power 4 5 5 4
Wet Mount PT: 2007A Specimen 3 • Evaluate micrographs 3-a, 3-b, and 3-c together as if different fields from the same patient • Identify cellular images labeled as: • 7 • 8 • 9 • Enter results on result sheet
7 8 Micrograph 3b – High Power 9 8
Wet Mount PT: 2007A Educational Challenge • Evaluate the following micrographs • These will not be graded!
Gram Stain – Clue Cell Nucleus
Gram Stain: Agents of BV Mobiluncus spp. Gardnerella vaginalis
Gram Stain:Squamous Epithelial & Clue Cell • These images are not typical of what you see in a wet mount – since you don’t do gram stains (but allow you to visualize the differences between normal epithelial cells and clue cells) • Squamous epi: a few gram positive bacteria (blue cells, typical of lactobacilli) are seen on the surface of the cell • Clue Cell: The surface of the cell is almost totally covered with blue gram positive bacteria. The cell nucleus is seen as a small red dot.
Gram Stain:Agents of Bacterial Vaginosis • Gardnerella vaginosis (small, gram negative [red] rounded rods - coccobacilli) • Mobiluncus sp. (small, slender gram negative [red] curved rods). • Both are normally found in the vagina (i.e., normal flora) • In bacterial vaginosis, these bacteria increase in number and degrade proteins in the vagina to form the amines that cause the characteristic unpleasant odor associated with BV.
Sign & Date Result Sheet • Testing Person • Site Coordinator • Send results to lab director within 10 days of electronic notification of PT challenge