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臨床生化 （ Ⅱ ） Instrumentational Analysis

臨床生化 （ Ⅱ ） Instrumentational Analysis. 賴滄海 教授 10-23-2007. Individual Steps In the Analytical Processes. Specimen acquisition Specimen identification Specimen delivery to laboratory Specimen preparation Specimen loading and aspiration On-analyzer specimen delivery. Ka = 【Hp -Ab】

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臨床生化 （ Ⅱ ） Instrumentational Analysis

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  1. 臨床生化（Ⅱ）Instrumentational Analysis 賴滄海 教授 10-23-2007

  2. Individual Steps In the Analytical Processes • Specimen acquisition • Specimen identification • Specimen delivery to laboratory • Specimen preparation • Specimen loading and aspiration • On-analyzer specimen delivery

  3. Ka = 【Hp -Ab】 【Hp 】【Ab】 Ka = affinity constant

  4. 免疫檢驗的分類 • 方式 Homogeneous Heterogeneous • 原理 Competitive Sandwich • 標誌物質 RIA FIA EIA CLIA

  5. Examples of IAs • Radio immunoassay (RIA) • Particle –enhanced turbidimetric inhibition IA (PETINIA) • Enzyme-linked Immunosorbent Assays (ELISA) • Enzyme multiplied Immunoassay Technique (EMIT) • Cloned enzyme donor IA (CEDIA) • Microparticle capture enzyme IA (M EIA) • Solid phase Fluorescence IA (SPFIA) • Particle concentration Fluorescence IA (PCFIA) • Fluorescence excitation transfer IA (FETI) • Substrate-level Fluorescence IA (FPIA) • Dissociation-enhanced lanthanide fluoro imnurco assay (DELFIA)

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