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FLUORESCENCE MICROSCOPY

FLUORESCENCE MICROSCOPY. IMMUNOFLUORESCENCE. Multiple immunofluorescence labelling of formalin-fixed paraffin-embedded (FFPE) tissue David Robertson, Kay Savage, Jorge S Reis-Filho and Clare M Isacke*

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FLUORESCENCE MICROSCOPY

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  1. FLUORESCENCE MICROSCOPY

  2. IMMUNOFLUORESCENCE

  3. Multiple immunofluorescence labelling of formalin-fixed paraffin-embedded (FFPE) tissue David Robertson, Kay Savage, Jorge S Reis-Filho and Clare M Isacke* Address: Breakthrough Breast Cancer Research Centre, The Institute of Cancer Research, 237 Fulham Road, London SW3 6JB, UK PUBLISHED 2008

  4. DUCTULES BREAST OF A PREGNANT MAMMAL

  5. MILK PRODUCTION LOBULE DUCTULE DUCT MILK SECRETION

  6. DUCTULE

  7. NUCLEUS OF SECRETORY CELL

  8. NUCLEUS OF MYOEPITHELIAL CELL

  9. SEM OF BREAST ALVEOLUS (OR ACINUS) SECRETORY CELL MYOEPITHIAL CELL FROM OUTSIDE ALVEOLUS FROM INSIDE ALVEOLUS

  10. BREAST SECRETORY CELL

  11. LIPID DROPLETS BEING SECRETED FROM THE SECRETORY CELLS OF BREAST DUCTULE EPITHELIUM LIPID DROPLET BEING EXTRUDED (IT IS COVERED IN PLASMA MEMBRANE) SECRETORY CELL MICROVILLI

  12. THE PLASMA MEMBRANE AROUND THE SECRETED FAT DROPLETS PREVENTS THEM FROM FLORMING CLUMPS IN THE MILK BUTTER BUTTER CHURN

  13. FOR COMBINED IMAGE OF MULTIPLE FLUORESCENT ANTIBODIES (DIIFERENT COLOURED FLUOROPHORES): PHOTOGRAPHS TAKEN WITH DIFFERENT EXCITATION LIGHTS MUST BE TAKEN AND THEN OVERLAPPED (“MERGED”)

  14. MYOEPITHELIAL CELL LUMEN OF DUCTULE NUCLEUS OF SECRETORY CELL INTERMEDIATE FILAMENTS OF SECRETORY CELLS ANTI- SMOOTH MUSCLE ACTIN ANTI-GLANDULAR CELL KERATIN DAPI (DNA-STAINING)

  15. DETECTION OF BREAST CANCER BY IMMUNOFLUORESCENCE THIS PARTICULAR CANCER IS DUE TO UNCONTROLLED GROWTH OF THE EPITHELIAL CELLS OF THE MILK DUCTS ANTIBODY AGAINSTESTROGEN RECEPTOR ANTIBODY AGAINSTSECRETORY CELL KERATINS CANCEROUS BUT NOT YET INVASIVE NON-CANCEROUS INVASIVE CANCER

  16. PEROXISOMES IN LIVER CELLS

  17. HeLA CELLS DAPI notice light and darker staining areas in the nucleus (HETEROCHROMATIN / EUCHROMATIN) Anti-ACTIN ANTIBODY

  18. BULL SPERM DAPI

  19. HeLA CELLS NUCLEUS BLUE; GOLGI BODIES GREEN;MITOCHONDRIA RED

  20. RAT URINARY EPITHELIAL CELLS Anti- CYTOCHROME OXIDASE ANTIBODY Anti- ACTIN ANTIBODY DAPI

  21. HeLa CELLS DAPI Anti - ACTIN Anti - TUBILIN

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