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Understanding Biotechnology: PCR, Restriction Enzymes & Gel Electrophoresis

Biotechnology, PCR, Restriction Enzymes, Gel Electrophoresis

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Understanding Biotechnology: PCR, Restriction Enzymes & Gel Electrophoresis

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  1. Biotechnology

  2. PCR (Polymerase chain reaction) • What is it?

  3. Restriction enzymes • What are they?

  4. Gel Electrophoresis • What is it? • How does it work? • DNA is isolated • DNA is copied with PCR • Cut with restriction enzymes • Run through charged gel box • Compared

  5. Comparing results

  6. GMO’s (Genetically modified organisms) • What are they? • How are they made? • Chosen gene is cut with restriction enzymes, leaving “sticky ends” • Circular bacterial DNA is cut with same restriction enzymes, leaving “sticky ends” • Cut pieces are joined together, so bacteria now produces protein in gene

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