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WT ap2c1 ap2c1/ complemented. 4. 4. 4. 4. 8. 8. 8. 8. 16. 16. 16. 16. 0. 0. 0. 0. min. post wounding. Kinase activity (MBP). MPK4. 4. 8. 16. 0. Protein (native Ab). 4. 8. 16. 0. min. post wounding. Kinase activity (MBP). MPK6.
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WT ap2c1ap2c1/ complemented 4 4 4 4 8 8 8 8 16 16 16 16 0 0 0 0 min. post wounding Kinase activity (MBP) MPK4 4 8 16 0 Protein (native Ab) 4 8 16 0 min. post wounding Kinase activity (MBP) MPK6 Protein (native Ab) Suppl. Figure 3: Comparison of wound activation of MAP kinases inWT, ap2c1 and ap2c1 complemented plants Wound-induced MPK4 and MPK6 activation in ap2c1 complemented plants is restored to the level of the WT. Kinase assays and western blot analysis were performed as in Figure 4A. Results shown were obtained on line #ap2c1-644.1 and are representative also for the results of line #ap2c1-644.2. Suppl. Figure 3