fish heath management lab 5 histology microanatomy l.
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Fish Heath Management Lab 5: Histology (microanatomy). Tarah Johnson March 2 nd , 2009. Objectives. Be able to microscopically identify cells and structures in the liver, kidney, and gills

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objectives
Objectives
  • Be able to microscopically identify cells and structures in the liver, kidney, and gills
  • Have the opportunity to microscopically examine histological sections of other fish organs (eyes, head, muscle, etc.)
liver
Liver
  • Hepatocytes
  • Hepatic Sinusoid
  • Central Vein and or Hepatic Portal Vein
  • Bile Duct
  • Arteriole
  • Glycogen Vacuole
slide4

Central Vein and Hepatic Sinusoids

Hepatic sinusoids

Erythrocytes visible within the central vein

400x

slide5

400x

400x

A

Bile Duct Arteriole

A. Glycogen Vacuole

Erythrocytes visible within arteriole

head kidney
Head Kidney
  • Interrenal gland
  • Chromaffin cell
  • Postcardinal vein
  • Hematopoietic tissue
slide7

400x

A

B

C

  • Interrenal Gland
  • Chromaffin Cell
  • Postcardinal vein
slide8

40x

A

B

A. Postcardinal vein

B. Hematopoietic tissue

trunk kidney
Trunk Kidney
  • Nephron (may not observe a complete nephron):

Renal corpuscle, proximal tube, distal tubule, and collecting duct

  • Renal Corpuscle:Glomerulus, Bowman’s capsule, basement membrane of glomerulus
  • Melanocytes
  • Collecting duct and/or Mesonephric duct
  • Connective tissue
slide10

400x

  • Renal Corpuscle
  • Proximal Tubule
  • Distal Tubule

C

B

A

slide11

400x

  • Glomerulus
  • Basement membrane of glomerulus
  • Bowman’s capsule

C

B

A

slide12

400x

C

A

B

  • Mesonephric Duct
  • Melanocytes
  • Connective tissue
gills
Gills
  • Filament
  • Lamella
  • Goblet (or mucous) cell
  • Chloride cell
  • Pillar (or pilaster) cell
  • Gill filament cartilage
slide14

Lamella

B

C

  • Filament cartilage
  • Pillar cell
  • Chloride cell

A

400x

Gill filaments and lamellae

40x

slide15

400x

Mucous or Goblet Cells

laboratory steps
Laboratory Steps
  • Use proper microscope handling procedures (distributed in Lab 2)
  • Examine histology slides of liver, kidney, and gills
  • Examine additional histology slides of other organs
be sure to
Be Sure to…
  • Clean oil immersion lenses
  • Clean slides and return to proper case (the same one you took it from)
  • Turn off microscopes and leave on low power objective when done