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# CHAPTER 28 Nested Designs - PowerPoint PPT Presentation

CHAPTER 28 Nested Designs. Tables, Figures, and Equations. From: McCune, B. & J. B. Grace. 2002. Analysis of Ecological Communities . MjM Software Design, Gleneden Beach, Oregon http://www.pcord.com. Example 1. Correct analysis (nested): MANOVA RICHNESS by WSHED(1,2) STREAM(1,4)

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Nested Designs

Tables, Figures, and Equations

From: McCune, B. & J. B. Grace. 2002. Analysis of Ecological Communities.MjM Software Design, Gleneden Beach, Oregon http://www.pcord.com

• Correct analysis (nested):

• MANOVA RICHNESS by WSHED(1,2) STREAM(1,4)

• /DESIGN WSHED VS 1, STREAM WITHIN WSHED = 1 VS WITHIN.

• Table 28.1. Nested sampling design analyzed with a univariate nested ANOVA.

Incorrect analysis (factorial):

MANOVA RICHNESS by WSHED(1,2) STREAM(1,4) /DESIGN.

In this example, the watersheds differ greatly, and there is little variation among streams within watershed. The results are again qualitatively similar between the correct and incorrect analysis (Tables 28.3 and 28.4).

Correct analysis (nested):

MANOVA RICHNESS by WSHED(1,2) STREAM(1,4)

/DESIGN WSHED VS 1, STREAM WITHIN WSHED = 1 VS WITHIN.

Table 28.3. Nested sampling design analyzed with a univariate nested ANOVA.

Incorrect analysis (factorial):

MANOVA RICHNESS by WSHED(1,2) STREAM(1,4) /DESIGN.

In this case, streams differ greatly (Table 28.5), but the factorial analysis detects an interaction between stream and watershed (Tables 28.6 and 28.7). The results from the two analyses differ greatly.

Table 28.5. Data set for Example 3.

• Correct analysis (nested):

• MANOVA RICHNESS by WSHED(1,2) STREAM(1,4)

• /DESIGN WSHED VS 1, STREAM WITHIN WSHED = 1 VS WITHIN.

• Table 28.6. Nested sampling design analyzed with a univariate nested ANOVA.

• Incorrect analysis (factorial):

• MANOVA RICHNESS by WSHED(1,2) STREAM(1,4) /DESIGN.

• Table 28.7. Nested sampling design incorrectly analyzed with a factorial ANOVA.

The method for adding more levels to the design is not immediately obvious in SPSS. In this case, we added to the correct, nested design another level "VALLEY," representing three different valleys within each watershed. Streams are nested within valleys. The syntax for requesting the analysis is:

MANOVA RICHNESS by WSHED(1,2) VALLEY(1,3) STREAM(1,4)

/DESIGN

WSHED VS 1,

VALLEY WITHIN WSHED = 1 VS 2

STREAM WITHIN VALLEY WITHIN WSHED = 2 VS WITHIN.

As in the other examples, variation at each level in design is compared with the variation in the next lower level.

Table 28.8. Nonparametric MANOVA of a Sørensen distance matrix, adapted from the example from Anderson (2001).

Table 28.8. Nonparametric MANOVA of a Sørensen distance matrix, adapted from the example from Anderson (2001).

Table 28.8. Nonparametric MANOVA of a Sørensen distance matrix, adapted from the example from Anderson (2001).

Table 28.8. Nonparametric MANOVA of a Sørensen distance matrix, adapted from the example from Anderson (2001).

Table 28.8. Nonparametric MANOVA of a Sørensen distance matrix, adapted from the example from Anderson (2001).