Protein Co-expression Service in E. coli System & Protein Co-expression in Mammalian Cells

1. Protein Co-expression Service in E. coli System & Protein Co-expression in Mammalian Cells Profacgen

2. Protein Co-expression Service in E. coliSystem Profacgen has investigated the use of E. coli co-expression techniques in protein manufacturing, and tried to extend from the common binary expression to the more complicated multi-expression. Results from various case studies have indicated co-expression in E. coli host to be a good alternative compared to endogenous purification or in vitro reconstruction from individually expressed components. It is also proved that co-expression of multiple proteins/complexes a useful alternative to the complicated and expensive expression systems, such as yeast, baculovirus or mammalian cell culture.

3. Co-expression of proteins that form a complex • Co-expression of subunits of a di- or multimeric protein • Co-expression of protein with chaperones and/or foldases

4. Profacgen offers a wide range of pharmaceutical services which includes biopharmaceutical product related impurities analysis. A variety of compounds added during the manufacture process of your biopharmaceutical products can appear in the final product as process- and product- related impurities. We provide you with effective process related impurities analysis, and product related impurity testing in biopharmaceuticals. As a result, we can help you to confirm the efficient removal of impurities by the downstream process and absence from the final product.

5. In addition, Profacgen can also assist the scientists with protein co-expression network analysis, a powerful approach to identify the tissues and the conditions in which important interactions occur. By integrating the co-expression data with a protein interaction network, the scientists will be able to determine the cellular conditions and tissue specificity of protein interaction network of human proteins.

6. Protein Co-expression in Mammalian Cells • Multi-protein assemblies can be found in a wide range of biological processes such as transcription, translation, RNA splicing, signaling cascades, protein transport, trafficking and degradation. After realizing that proteins do not work solo but interact extensively with one another as stable or transient complexes, life science researchers have directed their attentions to multi-protein-complexes for structural and functional studies.

7. With the vast base of isolation techniques and characterization technologies in our GLP / GMP compliant protein analysis laboratories, underpinned by over 20 years of experience in supporting biologic drug development and bioprocessing, we offer a full spectrum of ready-to-use analytical methods for the detection and quantification of process- and product-related residuals and impurities. Profacgen can ensure that you meet all regulatory expectations and specifications for your drug substance and drug product.

8. Profacgen provides several approaches for protein co-expression service. The easiest is to have multiple genes encoded by one vector or virus and co-transform or co-infect the host with several of them. Alternatively, each gene is encoded by a single vector or virus, either under the control of a single promoter or of several promoters, and a single transformation or infection is required. Profacgen offers unprecedented expertise and high quality custom co-expression service with competitive pricing. Request more information to see how Profacgen could be assistance.

9. Thanks for watching! Contact Profacgen • Address: 45-1 Ramsey Road Shirley, NY 11967, USA • Website: https://www.profacgen.com