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Anabolism: What micro-organisms do with all that 1. Reducing power 2.Energy 3. Raw building materials. Reducing Power. NADH/FADH glycolysis and Kreb’s cycle NADPH  oxidative photophosphorylation NADPH  anaerobic photophosphorylation 1. Reverse electron flow

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anabolism what micro organisms do with all that 1 reducing power 2 energy 3 raw building materials

Anabolism: What micro-organisms do with all that1. Reducing power2.Energy3. Raw building materials

reducing power
Reducing Power

NADH/FADHglycolysis and Kreb’s cycle

NADPH  oxidative photophosphorylation

NADPH  anaerobic photophosphorylation

1. Reverse electron flow

2. Protons from inorganic molecules (H2S, S2O3)

Protons leached from Fe-S containing molecules in PSI in

anaerobic photo-phosphorylation

energy atp
Energy--ATP
  • 1. Oxidation of organic molecules (glycolysis/Kreb’s cycle)
  • 2. Electron transport:
  • A. oxidative phosphorylation (PMF) chemiosmosis)—
  • oxygen the final proton/electron acceptor
  • B. anaerobic– inorganic molecules final electron acceptor
  • Oxidation of inorganic molecules (usually fed into electron
  • transport chain)
  • 4. Dissimulative reduction of inorganic molecules (CO2,
  • sulfides and iron
  • 5. Oxidative and anaerobic photophosphorylation
raw building materials
Raw building materials

Carbon/Nitrogen/Phosphorous etc. from the catabolism of

carbohydrates

lipids

proteins

nucleic acids

strange organic matter such as petroleum

Carbon from CO2 fixation

Nitrogen from Nitrogen (N2) fixation

Etc.

integrated pathway for catabolism and anabolism
Integrated pathway for catabolism and anabolism

CARBOHYDRATES

Ribose

LIPIDS

Glucose

A.A

Phosphoglycerate

Fatty

acids

A.A.

Kreb’s cycle

Phosphoenolpyruvate

A.A.

oxaloacetate

Pyruvate

a-keto

glutarate

succinate

Acetyl-CoA

Amino

acids

purines

pyrimidines

Amino

acids

NUCLEIC ACIDS

Amino

acids

PROTEINS

n.b. amino acids

(A.A)

one strange thing bacteria can eat for energy and biomass petrol
One strange thing bacteria can eat for energy and biomass—Petrol !!!

Mediated by oxygen and the

enzyme mono-oxygenase

variety of yeasts/ molds and

bacteria

another strange thing benzene toluene
Another strange thing—benzene/toluene

Requires oxygen, NADH. Aliphatic carbons are converted to linear

molecules that can be catabolized to succinate/Acetyl-CoA/pyruvate

which enter Kreb’s cycle—Pseudomonas spp.

slide8

CO2 fixation (Calvin cycle, reverse TCA cycle and hydroxy-proprionate cycle)How autotrophs reduce CO2 intomaterial they can use for biomass.

calvin cycle
Calvin cycle

In: CO2, NADPH and ATP

Out: Fructose-6 phos

intermediate of glycolysis

slide12
Nitrogen fixation: atmospheric nitrogen to fixed nitrogen that can be utilized by plants and bacteria
agricultural significance of nitrogen fixation
Agricultural significance of nitrogen fixation:

Crop rotations with plants that form intimate symbiotic relationships

with Rhizobium spp. (bacteria in root nodules)

peas, beans, soybean, alfalfa, clover

Pre seed watery environments with cyanobacteria (Anabaena azollae)

and the water fern Azolla.

Plant rice as rice plants grow they crowd out and kill the water fern

thus releasing the nitrogen that they require

pgs 685-692 in text book