Day 1, 23.9.2014 Download APE.exe and familiarize Look for Trypanosoma cruzi hexokinase in GenBank or in the TriTryp database
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Look for Trypanosomacruzihexokinase in GenBankor in theTriTryp database
synthesizeforwardand reverse oligonucleotides to amplifythewhole gene, insertingsuitablerestriction sites in the 5´and 3´ ends, start with BamH1 (5´) and Xho1 (3´) sites, but do not include thestopcodon.