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Multiplex Ligation-dependent Probe Amplification (MLPA ). For large deletion and deletion in unpredicted sites and duplication we can use MLPA In detection of aneupolidy e.g. trisomy 13,18 and 21 and monosomy turner. In this case probes are designed against STRs.

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multiplex ligation dependent probe amplification mlpa
Multiplex Ligation-dependent Probe Amplification (MLPA)
  • For large deletion and deletion in unpredicted sites and duplication we can use MLPA
  • In detection of aneupolidy e.g. trisomy 13,18 and 21 and monosomy turner. In this case probes are designed against STRs
multiplex ligation dependent probe amplification
Multiplex ligation-dependent probe amplification
  • Analysis by MLPA is a suitable alternative that is also capable of detecting new deletions and amplifications.
  • With MLPA, it is possible to perform a multiplex PCR reaction in which up to 45 specific sequences are simultaneously quantified.
  • Amplification products are separated by sequence type electrophoresis As only one pair of PCR primers is used,
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MLPA
  • MLPA reactions result in a very reproducible gel pattern with fragments ranging from 130 to 490 bp.
  • Comparison of this gel pattern with that obtained with a control sample indicates which sequences show an aberrant copy number