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Objectives??

Ex. 14: Skin Cultures and Importance of Selective and Differential Media for Isolating Gram-Positive Cocci. Objectives??. MSA: Selective and Differential Medium for Gram + Cocci.

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Objectives??

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  1. Ex. 14: Skin Cultures and Importance of Selective and Differential Media for Isolating Gram-Positive Cocci Objectives??

  2. MSA: Selective and Differential Medium for Gram + Cocci Composition: meat extract, casein, peptones; 7.5% NaCl, mannitolas only fermentable carbohydrate, phenol red indicator.

  3. Staphylococcus saprophyticuson MSA plate

  4. Day 1

  5. Control Organisms Skin Inoculum

  6. Day 2

  7. CatalaseTest H2O2 is by-product of aerobic respiration. Lethal to the cells.  Most aerobic organisms produce catalase for protection Catalase test differentiates among morphologically similar Gram+ cocci.

  8. Coagulaseactivates fibrin and leads to clot formation  Protective barrier around bacterial cells  Virulence factor • Test determines presence of coagulase enzyme • Used for differentiating S. aureus from other Staphylococci. CoagulaseTest

  9. Tube Coagulase Test Tests for bound of free coagulase check no later than 24 hours after inoculation!

  10. Slide Coagulase Test • Tests for bound coagulase • Positive if agglutination within 1 to 2 minutes

  11. the end

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