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Gene Regulation by Gal4-MEF2C and Gal4-NFATc3: Insights into Transcriptional Activation

Explore the synergistic effect of Gal4-MEF2C and Gal4-NFATc3 on gene expression, elucidating a 3-fold activation mechanism. Detailed analysis of truncated MEF2C and NFATc3 variants reveals their unique roles and interactions in regulating transcription. This study sheds light on the complex interplay between transcription factors in orchestrating gene expression for cellular processes.

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Gene Regulation by Gal4-MEF2C and Gal4-NFATc3: Insights into Transcriptional Activation

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  1. 5 - - pG5Luc - - 4 + - - + 3 Fold activation + + 2 1 0 - + + - - - - Gal4-MEF2C(2-473) - - - + - - - Gal4-MEF2C(2-117) + - - - - - - Gal4-MEF2C(117-473) - - - - + - - Gal4-MEF2C(2-57) - - - - - + - Gal4-MEF2C(2-57, 86-473) - - - - - - + Gal4-MEF2C(56-473) - + + + + + + FLAG-NFATc3 + CnA 5 pG5Luc 4 3 Fold activation 2 1 0 + + - Gal4-NFATc3(2-1110) - - + Gal4-NFATc3(2-314) - - - Gal4-NFATc3(314-732) - - - Gal4-NFATc3(732-1110) - + + MEF2C-FLAG Supplemental Fig 1 B A

  2. 40 35 30 1 58 87 473 25 TAD MADS MEF2 20 15 1 117 DE1 DE3 10 AdDNMEF2 myc LITR CMV GFP CMV 5 R24L 0 3xMEF2Luc MEF2A - - - - - - - - MEF2C1-117 - - - - - - - - MEF2C1-117R24L - - - - - - - - MEF2A1-131 + - + + + + + + + + + + + + + + + + + + + + Supplemental Fig 2 A B

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