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Analysis of Road Traffic and Gene Copy Numbers in Urban Areas

This study examines the relationship between urban road infrastructure and various gene copy numbers, specifically focusing on the Crenarchaeal 16S and amoA genes. It presents data visualized through multiple figures (S1, S3, S4, S5) with metrics measured in kilometers. The objective is to understand the interplay between urbanization and genetic diversity in microorganisms within these environments. The findings provide insights into microbial population dynamics, which may have implications for urban ecology and infrastructure planning.

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Analysis of Road Traffic and Gene Copy Numbers in Urban Areas

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  1. a b Highways 0 20 40 Other roads Urban area Kilometers Fig. S1

  2. 0 0 0 0 0 0 0 0 20 20 20 20 20 20 20 20 40 40 40 40 40 40 40 40 Kilometers Kilometers Kilometers Kilometers Kilometers Kilometers Kilometers Kilometers a c b 9.0 103 3.5 104 2.7 105 7.0 103 2.5 104 1.3 105 5.0 103 0 20 40 1.5 104 7.5 104 3.0 103 0.5 104 1.0 103 2.5 104 Kilometers d e f 9.0 103 2.7 104 7.0 103 7.0 103 1.7 104 5.0 103 5.0 103 1.2 104 3.0 103 3.0 103 7.5 103 1.0 103 1.0 103 2.5 103 g h i 2.2 103 1.7 103 9.0 104 5.0 104 1.2 103 5.0 104 3.0 104 7.5 102 1.0 104 1.0 104 2.5 102

  3. 105 104 Crenrachaeal 16S gene copy ng-1 DNA 103 102 10 102 103 104 105 Crenarchaeal amoA gene copy number ng-1 DNA Fig S3

  4. 0 0 0 0 0 0 0 20 20 20 20 20 20 20 40 40 40 40 40 40 40 Kilometers Kilometers Kilometers Kilometers Kilometers Kilometers Kilometers a b c 0.11 0.045 0.07 0.35 0.035 0.05 0.025 0.25 0 20 40 0.03 0.015 0.15 Kilometers 0.01 0.05 0.005 d e f 0.009 0.27 0.0175 0.007 0.17 0.0125 0.005 0.12 0.0075 0.003 0.075 0.025 0.001 0.0025 g h Fig. S4 2.75 450 2.25 350 1.75 250 1.25 150 0.75 50 0.25

  5. a c b 0.285 0.0048 0.0058 0 20 40 0 20 40 0 20 40 0.059 0.0009 0.0017 Kilometers Kilometers Kilometers Fig. S5

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