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Background

Development of a Phosphate Biosensor for Soil and Ground Water [PO 4 3- ] Detection Serkan Akar Florida International University/Applied Research Center Dept. of Biomedical Engineering Master’s Student. Background.

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Background

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  1. Development of a Phosphate Biosensor for Soil and Ground Water [PO43-] DetectionSerkan AkarFlorida International University/Applied Research CenterDept. of Biomedical Engineering Master’s Student

  2. Background • The recent research shows that stored uranium in Hanford causes extensive contamination in the soil and ground water. • Phosphate PO43- reacts with Uranium and reduces it from U6+ to U4+. (Stabilization of Uranium) • The detection/quantification of [PO4] is essential.

  3. Method • Develop an enzymatic biosensor which will carry out the reaction and produce H2O2. • The produced H2O2is detected via electro-chemical methods. • The concentration of [PO4] is then calculated from the calibration curve obtained as a result of addition of PO4 in the solution.

  4. Reaction Pyr+PO4+O2 POX Acetyl P + H2O2 + CO2

  5. Detection Mechanism • The reaction produces H2O2 which can be electro-chemically detected. • H2O2 2H+ + 2e- + O2 • The Sensing Response is obtained from oxidation current of above reaction

  6. Experimental Setup

  7. Experimental Setup

  8. CV Background Buffer Solution

  9. Amperometry Background Current Time DCPA of The Buffer

  10. Graphs of H2O2 PO43- Addition Points

  11. Normalized Data

  12. Conclusion • Obtained data clearly indicates that PO4 can be detected and measured/quantified successfully by the designed system.

  13. Acknowledgement • Dr. Leonel Lagos (DOE Fellows Program Director) • Dr. Vekalet Tek ( ARC Mentor) • Dr. Thomas G. Thundat (ORNL Mentor)

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