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Chapter 6. DNA Structure and Function Part 3. Cloning. Cloning means making an identical copy of something In biology it can refer to A lab method in which researchers copy fragments of DNA A reproductive method in which researchers produce an exact genetic copy of an entire organism.

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chapter 6

Chapter 6

DNA Structure and Function Part 3

cloning
Cloning
  • Cloning means making an identical copy of something
  • In biology it can refer to
    • A lab method in which researchers copy fragments of DNA
    • A reproductive method in which researchers produce an exact genetic copy of an entire organism.
cloning1
Cloning
  • Nature makes genetically identical organisms all the time by:
    • Asexual reproduction
    • The splitting of an embryo
  • Artificial twinning is reproductive cloning performed by researchers in a lab.
  • In this process, a ball of cells is grown in a lab from a fertilized egg.
  • This ball is then forced to separate into two identical halves, each of which then develops as a separate embryo.
  • These embryos are then implanted into surrogate mothers, who give birth to identical twins.
  • This technique is performed often in research laboratories with animals.
cloning2
Cloning
  • Since nature’s clones, identical twins, get their DNA from two parents (who are genetically different from one another), even though they are identical to each other, they are not identical to either parent.
  • On the other hand, if an animal breeder wants to produce an exact copy of a specific, adult organism, they perform adult cloning, another type of reproductive cloning.
  • In adult cloning, the researcher begins with a single body cell taken from an adult organism.
cloning3
Cloning
  • The trick to adult cloning lies in the fact that, although all adult body cells contain the same DNA (for the most part), these cells have differentiated into certain cell types (such as a liver cell or muscle cell) due to the fact that they have begun using certain subsets of their DNA.
  • This differentiation is a one-way path.
  • Once a cell has specialized (or differentiated), all of the cells that are produced from that cell will be specialized in the same way.
  • For example, once a cell has become a liver cell, it can only produce other liver cells. All of its DNA has been turned “off” except for the subset that codes for the cell to be a liver cell.
cloning4
Cloning
  • Therefore, in order to clone an adult using one of these specialized body cells, scientists must first transform this differentiated cell an undifferentiated cell by turning its unused DNA back “on”.
  • In somatic cell nuclear transfer (SCNT), a researcher removes the nucleus from a unfertilized egg, then inserts into this “empty” egg a nucleus from an adult body (somatic) cell.
  • If things go as planned, the egg’s cytoplasm induces the DNA from the somatic cell to induce the development of an embryo.
  • This embryo is then transplanted into a surrogate mother.
  • The animal that is born from this process with be genetically identical to the animal that donated the nucleus.
cloning6
Cloning
  • Dolly the sheep was the first mammal successfully produced as a result of adult cloning using SCNT.
cloning7
Cloning
  • Adult cloning is now a common practice amongst those who breed prized animals such as livestock.
  • Benefits of adult cloning of animals include producing more offspring in a given time frame than can be produced by traditional breeding methods and producing offspring from an animal that has been castrated or has even died.
cloning8
Cloning
  • The controversy that lies with adult cloning is not really about cloning animals, but the fact that, now, human cloning is within the realm of possibility.
  • In fact, researchers are already using SCNT to produce human embryos for research, a process known as therapeutic cloning.
  • Researchers harvest stem cells from the cloned embryos.
  • Even though reproductive cloning of humans is not intent of this research, it brings us one closer to that end.
the human cloning debate
The Human Cloning Debate
  • Please visit this website and complete the graphic organizer on the next slide. Print your completed diagram and bring with you to class.
  • http://library.thinkquest.org/24355/data/reactions/proconmain.html
  • Be sure to visit each topic on this website:
    • PRO
    • CON
    • VIEWS
    • Cloning’s Future
    • Misconceptions
    • Regulation
    • Don’t limit your search to just what’s listed on this site. This site is just a start.
    • Visit at least two other reputable websites to gather information and turn those websites in with your diagram when asked to do so.
slide13

PROS

CONS