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Restriction Mapping

Restriction Mapping. Restriction Mapping: Double Digests. Factor I – Single Cut. Factor 2 – Single Cut. Restriction Mapping: Double Digests. Restriction Mapping: Double Digests. Restriction Mapping: Double Digests. Uses of Restriction Mapping. Fingerprinting

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Restriction Mapping

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  1. Restriction Mapping

  2. Restriction Mapping:Double Digests Factor I – Single Cut Factor 2 – Single Cut

  3. Restriction Mapping:Double Digests

  4. Restriction Mapping:Double Digests

  5. Restriction Mapping:Double Digests

  6. Uses of Restriction Mapping • Fingerprinting • Restriction Fragment Length Polymorphism Mapping

  7. Cloning

  8. pUC19

  9. pUC19 Multiple Cloning Site

  10. AGCT PLASMID TCGA AGCT KAN TCGA Ligation

  11. AGCT PLASMID TCGA AGCT KAN TCGA Ligation DNA ligase

  12. AGCT PLASMID TCGA AGCT KAN TCGA Ligation

  13. AGCT PLASMID TCGA AGCT KAN TCGA Ligation

  14. Ligation

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