Plant/Microbe Interactions - Beneficial. Interactions involving plant roots Rhizoplane - the surface of the plant root, root hairs present large surface area (> 6 m 2 for an average wheat plant). Only 4 - 10% of the rhizoplane is in direct contact with soil microbes.
Colonization of a root by an endomycorrhizal fungus
(Brundrett et al. 1985 Can. J. Bot. 63: 184).
a. Spores of (i) Gigaspora, (ii) Glomus, (iii) Entrophospora, and (iv) Acaulospora; b. germinating spore; c. hyphal network and spores; d. hypha and spores around root; e. hyphal penetration inside root; f. intracellular arbuscules; g. intraradical vesicles; h. colonized plant
- Isolation of AMF pure culture strain : single spore
- Choice of a host plant : Allium porrum, Sorgum bicolor, Zea Mays, Paspalum otatum
a. Isolated spores; b. germinating colonized root segment; c. carrot root in culture; d. AMF root-organ culture; e. closer view of an AMF root-organ culture
a. Seeding mycorrhizal substrates; b. mycorrhizal seedling production; c. growth chamber inoculum propagation; d. root growth and colonization; e. colonized seedlings; f. field inoculum propagation