Plant Tissue Culture. What Is plant tissue culture ?. Or in vitro culture? Or in vitro propagation? Or Micropropagation ?. Definition. the culture of plant seeds, organs, explants, tissues, cells, or protoplasts on nutrient media under sterile conditions.
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Or in vitro culture?
Or in vitro propagation?
Or Micropropagation ?
the culture of plant seeds, organs, explants, tissues, cells, or protoplasts on nutrient media under sterile conditions.
Usually, the younger, less differentiated the explant, the better for tissue culture
1. Different species show differences in amenability to tissue culture
2. In many cases, different genotypes within a species will have variable
responses to tissue culture; response to somatic embryogenesis has
been transferred between melon cultivars through sexual
Shoot tip - Auxins
Roots - water, vitamins
mineral salts and cytokinins
the potential or inherent capacity of a plant cell to develop into an entire plant if suitable stimulated.
It implies that all the information necessary for growth and reproduction of the organism is contained in the cell
The capacity of mature cells to return to meristematic condition and development of a new growing point, followed by redifferentiation which is the ability to reorganize into new organs
the endogenous potential of a given cell or tissue to develop in a particular way
1. shoot tip culture
2. Root culture
3. Leaf culture
4. anther culture
+ Auxin leads to high [Putrescine]
Single cells sloughed
off the surface
Development and cycling
of Pro-embryonic masses
E.g. Carrot, Monocots, some conifers
A general phenomenon of all plant regeneration systems that involve a callus phase
2n = 2X = 14
2n = 2X = 14
2n = X = 7
H. Bulbosum chromosomes eliminated
enzymic digestion with pectinase
Removal of large cell aggregates by sieving.
Plating of single cells and small cell aggregates - only viable cells will grow and can be re-introduced into suspension.Introduction of callus into suspension
Sieve out lumps
Development of GM foods
Genes for crops
Benefits of GM crops, especially in developing countries
How to get genes into cells to give transformed cells
How to get a plant back from a single transformed cellPlant genetic engineering
Crown gall from Agrobacterium