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  1. Background Demographic: • Undergraduate Students in Pre-Lab Project: • Streaming On-line Biology Video pieces: One per Preceptor, per year. • Individual Video Breakdown: • Intro: Host, Dr. Robert Lue • Faculty Spotlight: Harvard Professor in the particular field of lab and study • Lab/Pre-Lab: Actual Hands On Demonstration of Lab of Topic • Outro: Host, Dr. Robert Lue • Mandatory Feed Back Form (Quiz) • Browser/Monitor: • Mac/PC; Quicktime/Flash

  2. “Players” Executive Producer: • Robert Lue 5-9924 Associate Producer: • Alain Viel 6-5606 Director of Photography/Production Manager: • Leigh Stimolo 6-7093 Content Supervisor/Coordinator: • Christine Rodriguez 203-665-6434 Writers: • Cheryl Vaughan 401-383-5968 • Christine Rodriguez 203-665-6434 • Tom Torello 6-1206 • Erica Pantages 4-7925 • Marion Shonn TBA • On-Location Audio Engineer: • Jack Howard 5-4035 Videographers/Editors: • Lansing Wagner 5-2683 • Steve Keirstead 5-2683 • Leigh Stimolo 6-7093 Production Assistants: • Eliza Stephens 5-8258 • Clark Magnan 5-8258 Voice Over Studio/Engineer: • Bill Countie, Media Production Center 5-9440 Animator: • Matt Bohan, Freelance Flash Animator • A/V Multimedia Developer: • Leigh Stimolo 6-7093 Video Server Network Administrators: • Webmaster: Steve Keirstead 5-2683 • Chiasm Server: Pepe Lopez 6-8479

  3. Pre-production Deliverables Choose Lab Topic Create Outline Storyboard Lab Video Sequence Script Lab Video Sequence Begin Location Scouting Research and Submit Talent Selection Script Rewrite Talent Rehearsals Shot List Breakdown Book Location Book Talent Book Crew Recruit Production Assistants Finalized Script with Shot Sequence 1-2 Day Lab Shoot Collect Signed Talent Release Forms Collect Master Tapes & Label Hand off Master Lab Video Tapes to Editor Select Faculty member for Spotlight

  4. Pre-production Deliverables Compose Ten Faculty Spotlight Questions Submit Questions Optional Presence at Faculty Spotlight Shoot Compose a small 1-2 paragraph Intro and Outro script Compose Evaluation Questions for Quiz

  5. Production Deliverables Lab Video Sequence Shoot— Audio: Jack Howard Video: Steve Keirstead (BS 54) and Lansing Wagner (BS 50/BS 52) Lighting: Leigh Stimolo Faculty Spotlight Shoot— Audio: Jack Howard Video: Leigh Stimolo Lighting: Leigh Stimolo Interviewer: Robert Lue Intro/Outro Shoot— Audio: Jack Howard Video: Leigh Stimolo Lighting: Leigh Stimolo

  6. Post-Production Deliverables Animation Sequences— Animation Script Animation Storyboard Animator: Matt Bohan Lab Video Sequence Editing— Video Editors: Lansing Wagner (BS 50 & BS 52) and Steve Keirstead (BS 54) Line Producer: Leigh Stimolo On-line Compressionist: Leigh Stimolo Voice Over (VO)— Voice Over Script VO Studio: Bill Countie, Media Production Center User Interface— Developer: Leigh Stimolo Web site— Developer/Programmer: Leigh Stimolo

  7. BS 50: Topics BS50 • Sterile techniques (basics, plating microorganisms, titers and dilutions, mammalian cell culture) • Chip analysis (techniques, SNPs/pharmacogenomics, transcriptional profiling, limitations of data) • Meiosis and tetrad analysis (Meiosis animation, Neurospora vs Saccharomyces, tetrad dissection vs random sporulation) • Use of reporters (B-galactosidase, GFP, others) • Northerns and Southerns • Cloning, transformation, and transfection

  8. BS 52: Topics BS 52 • Electrophoresis (agarose vs acrylamide, what %, what Amp/Volt to run at, IEF, capillary) • DNA sequencing (Restriction enzyme mapping, lab/gel techniques, methods such as PCR, Sanger, automated, etc.) • DNA topology (1D/2D gels, animation of enzymatic reaction, run on gel) • Gel shift/analysis of DNA binding proteins • Staining and labeling (histology/cells, DNA, types of stains used, when and why they work, radioactivity, enzymatic assays) • FISHhttp://www.courses.fas.harvard.edu/~bs52

  9. BS 54: Topics BS54 • Centrifugation (vocabulary, techniques, types of rotors, supernatant vs pellet, from microfuge to Sorvall to ultra) • Generation of antibodies (polyclonal vs monoclonal, modified secondary antibodies) • Beads and Tags (biochemical sorting, IP/Co-IPs, GST, 6-His, cell sorting, agarose beads, etc.) • Gradients (refer to ultracentrifugation, subcellular fractionation, how to get molecular materials, labelled organelles?, DNA/CsCl gradient, proteins such as ribosomes, etc) • Protein sequencing and analysis (old vs new methods) • ELISAhttp://www.courses.fas.harvard.edu/~bs54

  10. BS 50: Year 1 BS50 : Preceptor, Christine Rodriguez (Rich Dearborn, Adam Fagen) • MICROARRAY, Chip Analysis On-Screen Pre-Lab Talent: • Claire Bailey Voice Over Talent: • Claire Bailey (NYR) Faculty Spotlight: • TBD, possibles: Michael Laub, Hans Hoffman (NYR) Other Content and Production Assistants: • Undergraduate Student: Joo Hee & Allison

  11. BS 52: Year 1 BS52 : Preceptor, Kenna Mills (Lauraine Dalton) • DNA SEQUENCING On-Screen Pre-Lab Talent: • Dion Dickman Voice Over Talent: • Jan or Dion Dickman (NYR) Faculty Spotlight: • Dan Branton Other Content and Production Assistants: • Undergraduate Student: N/A

  12. BS 54: Year 1 BS54: Preceptor, Cheryl Vaughan • ANALYZING PROTEINS On-Screen Pre-Lab Talent: • John Neven, Emy Chen, Darcy Mootz Voice Over Talent: • Darcy Mootz Faculty Spotlight: • TBD, possibles: Steve Gygi (NYR) Other Content and Production Assistants: • Undergraduate Student: N/A

  13. BS 50: Year 2 BS50 : Preceptor, Christine Rodriguez (Erica Pantages) • NORTHERN/SOUTHERN, Cloning On-Screen Pre-Lab Talent: • TBA (NYR) Voice Over Talent: • TBA (NYR) Faculty Spotlight: • TBD, possibles: TBA (NYR) Other Content and Production Assistants: • Undergraduate Student: TBA

  14. BS 52: Year 2 BS52 : Preceptor, Tom Torello • TBD On-Screen Pre-Lab Talent: • TBA (NYR) Voice Over Talent: • TBA (NYR) Faculty Spotlight: • TBD, possibles: TBA (NYR) Other Content and Production Assistants: • Undergraduate Student: TBA

  15. BS 54: Year 2 BS54: Preceptor, Cheryl Vaughan (Marion Shonn) • ANTIBODIES On-Screen Pre-Lab Talent: • TBA (NYR) Voice Over Talent: • TBA (NYR) Faculty Spotlight: • TBD, possibles: TBA (NYR) Other Content and Production Assistants: • Undergraduate Student: TBA

  16. YEAR TWO emails Robert Lue lue@fas.harvard.edu Leigh Stimolo stimolo@fas.harvard.edu Jack Howard jhoward@fas.harvard.edu Alain Viel aviel@fas.harvard.edu Cheryl Vaughan cdvaugh@fas.harvard.edu Christine Rodriguez christinerodriguez@post.harvard.edu Lansing Wagner lwagner@fas.harvard.edu Steve Keirstead keirst@fas.harvard.edu Kenna Mills kmills@fas.harvard.edu Tom Torello torello@fas.harvard.edu Erica Pantages pantages@fas.harvard.edu Marion Shonn mshonn01@granite.tufts.edu Eliza Stephens estephen@fas.harvard.edu Clark Magnan cmagnan@fas.harvard.edu

  17. Equipment: Camera/Audio Shotgun Microphone Fishpole Canon XL-1S Mini DV Camcorder FP-33 Stereo Mixer Lavalier Microphone

  18. Equipment: Lights Arri: 300 Watt Fresnel Tungsten Light Tota Light Kit with Umbrellas & Stands SoftBox (Chimera)