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The Impact of Climatic Factors on Fecal Coliforms/Fecal Streptococci Ratio Variability. Kellie E. Jones Longwood College Department of Natural Sciences Coauthor: Genii A. Grimsley Faculty Advisor: Dr. David Buckalew. Fecal Coliforms Vs. Fecal Streptococci. FC/FS Source of Pollution.

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the impact of climatic factors on fecal coliforms fecal streptococci ratio variability

The Impact of Climatic Factors on Fecal Coliforms/Fecal Streptococci Ratio Variability

Kellie E. Jones

Longwood College

Department of Natural Sciences

Coauthor: Genii A. Grimsley

Faculty Advisor: Dr. David Buckalew

fc fs source of pollution
FC/FSSource of Pollution

Gerba, Charles P., et. al. Environmental Microbiology. New York: Academic Press,1999.

research hypothesis
Research Hypothesis

FC/FS ratio

  • During moderate climatic conditions variability decreases
  • Enhances the application of the ratio as a bacterial source indicator
method
Method
  • Collection of water samples
  • Presumptive test for fecal streptococci
  • Plate count
  • Confirmatory tests for fecal streptococci
presumptive test
Presumptive Test
  • Membrane Filtration
  • KF Streptococcal Broth
  • Incubate at 37º +/- 0.5° C for 48 +/- 2 hrs
  • Perform Plate Count
  • Calculate FC/FS ratios
confirmatory test
Confirmatory Test
  • Bile Esculin Agar
  • Incubate at 44.5º C +/- 0.5° C for 18 hrs
  • Observe Growth
t test results for november march vs september and october
T test Results for November – March vs. September and October

Ang 17.0 1.510 0.25>p>0.10

App 1.0 1.281 0.50>p>0.25

App 2.0 0.529 1.00>p> 0.50

Say 5.0 1.560 0.25>p>0.10

Say 8.0 1.237 0.50>p>0.25

Vau 14.0 1.369 0.25>p>0.10

t test results for november march vs september october and april 1
T test Results for November – March vs. September, October, and April (1)

Ang 17.0 1.2607 0.50>p>0.25

App 1.0 0.9843 0.50>p>0.25

App 2.0 0.8104 0.50>p>0.25

Say 5.0 1.4487 0.25>p>0.10

Say 8.0 1.3701 0.25>p>0.10

Vau 14.0 1.1762 0.25>p>0.10

t test results for november march vs september october and april 2
T test Results for November – March vs. September, October, and April (2)

Ang 17.0 1.77 0.25>p>0.10

App 1.0 0.977 0.50>p>0.25

App 2.0 0.8104 0.50>p>0.25

Say 5.0 1.8448 0.25>p>0.10

Say 8.0 1.3701 0.25>p>0.10

Vau 14.0 1.424 0.25>p>0.10

discussion
Discussion
  • Already see a shift in p values with the addition of April’s data
  • With more research the critical value could possibly be reached and there may actually be less variability in moderate months
  • Monthly variation in temperature and precipitation counteract the reliability of the FC/FS ratio
conclusion
Conclusion

We feel that it is important to continue

research on the FC/FS ratios because

they are the most inexpensive source

indicators of non-point bacterial

contamination in waterways.