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Detection of Single Red Blood Cell Magnetic Property using a Highly Sensitive GMR-SV Biosensor. Sang-Suk Lee, Sang-Hyun Park Kwang-Suo Soh 2006.9.27 CKC Symposium. Contents. Magnetism and Sensitivity New Functional Soft Magnetic Materials Measurement and Resolution

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detection of single red blood cell magnetic property using a highly sensitive gmr sv biosensor

Detection of Single Red Blood Cell Magnetic Property using a Highly Sensitive GMR-SV Biosensor

Sang-Suk Lee, Sang-Hyun Park

Kwang-Suo Soh

2006.9.27 CKC Symposium

contents
Contents
  • Magnetism and Sensitivity
    • New Functional Soft Magnetic Materials
    • Measurement and Resolution
  • Red Blood Cell Magnetophrosis
    • Oxygen - RBC
    • Magnetic Susceptibility
    • Magnetophoretic Mobilities
  • Set up of Measurement System
    • Micro Capillary Technology
    • Optical Tweezer Technology
  • Further Corporation Environment
    • Research Field of Prof. Tony Bland’s Group
    • Future Research Plans
metals spin polarization p and magnetism
Metals, Spin polarization (P), and Magnetism

Metal : n() = n() ( P = (n()-n())/(n()+ n()) =0 )

Ferromagnetism : ( 0<P <1 )

Half Metals: CrO2, Fe3O4, PtMnSb (P = 1)

3d 10-x

4f 14-x

slide5

Properties of GMR-SV Multilayers

Ta 5 nm

Hc

FM (Free Layer)

NiFe 10 nm

NFM (Spacer)

Cu 2.6 nm

FM (Pinned Layer)

NiFe 4.0 nm

AFM (Pinning Layer)

  • M-H curve

FeMn 7.0 nm

Ta 5 nm

Rap

Sensing

position

Rp

Rp

  • M-R curve

MR Ratio  (Rap-Rp)/Rp = 4 ~ 9 %

Magneto sensitivity MR/H

slide6

Advantage of GMR-SV Biosensor

  • The low requirement for sample amount
  • Easy integration for multianalyte detection on a single chip
  • Inexpensive and portable devices requiring little or no expertise
  • for their use

Application of GMR-SV Biosensor

Silica coated magnetic nanoparticles

Replace by RBC

PR(1.3 um)

SiO2(100 nm)

Contact pad (160 nm)

SV Sensor

Silicon substrate

highly sensitive magnetic films
Highly Sensitive Magnetic Films
  • Ni77Fe14Cu5Mo4

(Conetic film (Mu-metal))

  • Optimized condition : Hc = 0.055 Oe
  • Minimized purpose : ~0.055 Oe

(predicted values)

MS(MR/H) = 50 ~150 %/Oe

  • One of several hundreds for Hc of NiFe

Hc = 5~10 Oe

MS(MR/H) = 0.5 ~1.5 %/Oe

  • Measurement by using SQUID
    • Sensitivity - nano tesla (10-9 T)

=> 10-5 Oe

    • NiFe, NiFeCo => 10-2~10-3 Oe
    • NiFeCuMo => 10-4~10-5 Oe

(theoretically 10-6)

slide8

Expectation of a Very High Sensitivity of GMR-SV

Earth field

Electric Instruments

around field

Cosmos Magnetic field

High Volt

Transmitter,

Transformer,

Choke Coil,

Motor

Bio-magneto signal

General & Super-

Conductor Magnet

Magnetic field measuring limit

EEG

ECG

Permanent

Magnet

Tesla

Sensitivity of GMR/SV Biosensor

  • Sensor size : 26 m2
  • Output : 100 V , Resolution : 100 nT = 10-3 G
  • M = 510-22 emu (erg/G)  5 10-2 B
slide9

The Hemoglobin Properties

Of Red Blood Cell

deoxyhemoglobin

methemoglobin

oxyhemoglobin

  • Ferrous iron(Fe2+)
  • Fe2O3
  • Binding Oxygen
  • Molecules
  • 2-pair Polypetide Chain
  • Globin+4 Heme Group
  • Ferric iron(Fe3+)
  • Fe3O4
  • Loss of carrier power of oxygen and carbon dioxide
  • Blue-green color

* RBC : normal adult blood volume = 46 L

average number = 45×106/cc

circulatory lifetime = 120 days

1 RBC = 3×106 Hemoglobin

1 Hemoglobin = 4 Fe atoms

slide10

Ligand & Light Absorption Hemoglobin and Fe

 Diamagnetic Properties

 Paramagnetic Properties

slide11

Red Blood Cell

Magnetophoresis-1

1.Capillary magnetophoresis of Human blood cells

trapping in a flow systemJ. of Chromatography A, 2002

Apparatus

Results

slide12

Red Blood Cell

Magnetophoresis-2

2. Red Blood Cell Magnetophrosis

Maciej Zborowski et al, Biophysical Journal 84, 2638 (2003)

  • The measured magnetic moments of hemoglobin :
  • its compounds on the relatively high hemoglobin concentration of human erythrocytes
  • 2) Differential migration of these cells was possible
  • if exposed to a high magnetic field (1.40 T).
  • 3) Development of a new technology, cell tracking velocimetry (CTV) the migration velocity of oxy-, deoxy-, and metHb-containing erythrocytes
slide13

Red Blood Cell

Magnetic Susceptibilities

slide14

Red Blood Cell

Magnetophoretic Mobilities

slide15

Detection of Magnetic Nanoparticles

Ring Pattern by Liquid Drop Motion of Nano-particles

Before drop

After drop :

formation of

ring pattern

slide16

Output Sensing Signal Observation of Nanopartices

Change of Sensing Position by the abrupt Variationof Magnetic Field

Drop point

Before state : max & min signal

slide17

Capillary Capture

Red Blood Cell

Biophysics of cell membranes :

Investigation of the changes in the mechanical and

rheological properties of blood cells in diabetes

Taken by http://newton.ex.ac.uk/research/biomedical/membranes/

slide21

Micro-hole Capillary with RBC

and Biosensor

Pure-RBC

26 m2

slide22

Capillary and Approach to Biosenor

GMR-SV Biosensor

Red Blood Cells

Capillary

Red Blood Cell

slide23

Micro-capillary Moving

and Manipulating Images

Needs and supplememts:

Advanced Microscope, CCD Images, Uptaking RBC Techniques

slide24

Biological Cell Detection using

Ferromagnetic Microbeads {by T. Bland’ Group}

slide25

Integrated microfluidic cell with multilayer ring sensors for single magnetic microbead detection {by T. Bland’ Group}

slide26

Future Research Plans

To obtain an analytic value of bio-magnetic molecules

such as : RBC, Hemo-Sanal, etc

Using : (1) Micro-capillary controlling technology

(2) Optical tweezer trapping and manipulation

Fabrication of high sensitive GMR/SV biosensor

Fabrication of a highly sensitive GMR/SV biosensor with conetic film

Extraction of RBC or Hemo-Sanal from Bonghan Duct

Extraction of RBC or Heme-Sanal from Bonghan Duct

Investigation of single RBC’s and

Hemo-Sanal’s magneto-properties

< Dec. 2006

 Feb. 2007 >

BPL, SNU,

CKC Research

< Dec. 2006

 Feb. 2007 >

Nano-bio Lab.

Sangji University

< Sept. 2006

 Nov. 2007 >

Practical use of biosensor and

medical instruments

Set up measuring system,

using micro-capillary and optical tweezer