Benefits of scientific cooperation among European countries in medical research

1. Benefits of scientific cooperation among European countries in medical research Daniela Jezova

2. Institute of Experimental Endocrinology Slovak Academy of Sciences Bratislava

3. Project of the Centre of Excellence supported by European Union Title of the Project „Reduction of Negative Impact of Environmental Factors on Human Health“ Co-ordinator: Assoc.Prof. Daniela Ježová

4. STRESSNUTS Institute of Experimental EndocrinologySlovak Academy of SciencesBratislava, Slovakia Coordinator: Assoc.Prof. Daniela Ježová Main „mission” of this Centre To provide experimental and clinicaldata leading to specific recommendations aimed atreducingthe negative impact of environmental factors on human health

5. Main positive achievements • obtaining high quality scientific data with relevance to clinical research • significant improvement of the training process of young researches both from EU and Slovakia • active and broad cooperation among European scientists

6. Main positive achievements • obtaining high quality scientific data with relevance to clinical research • significant improvement of the training process of young researches both from EU and Slovakia • active and broad cooperation among European scientists

7. placebo lamotrigine Most important scientific result (one selected from many) STRESSNUTS Evidence has been obtained to support further clinical research on the following substances to modulate hormone release and treat the negative impact of demanding stressful situations, anxiety and depression: mood stabilizers extract of Ginkgo biloba pentoxifylline antidepressants selected amino acids

8. Public speech Prolonged anticipation Prolonged speech Emotional topics Video recording of speech Standing during speech Use of microphone Bright light Open space

9. Amino acid supplementation

10. Serotonine and stress

11. Enhancement of stress-induced ACTH release by treatment with citalopram and tianeptine insulin

12. obtaining high quality scientific data with relevance to clinical research • significant improvement of the training process of young researches both from EU and Slovakia • active and broad cooperation among European scientists

13. Long-term stays in the Centre „Postdoctoral fellow“ Sven Mostböck, PhD., Austria Laboratory Chief: Ing. J. Brtko, PhD. „Postdoctoral fellow“ Silvia Anette Wein, PhD., Germany Laboratory Chief : Ing. E. Šeböková, DSc. „Postdoctoral fellow“ Ibolya Bodnár, PhD., Hungary Laboratory Chief : RNDr. R. Kvetňanský, DSc.

14. Long-term stays in the Centre „Senior Visitor“ Prof. Barbro B. Johansson, M.D., Sweden

15. Long-term stays in the Centre „PhD. Student“ Aikaterini Makatsori, M.D., Greece Tutor : Assoc. Prof. D. Ježová

16. List of publications of the PhD student A. Makatsori, M.D. • Makatsori A., Duncko R., Moncek F., Loder I., Katina S., Jezova D.:Modulation of neuroendocrine response and non-verbal behavior during psychosocial stress in healthy volunteers by the glutamate release inhibiting drug lamotrigine. Neuroendocrinology 79:34-42, 2004 • Makatsori A., Duncko R., Schwendt M., Moncek F., Johansson B. B., Jezova D.: Voluntary wheel running modulates glutamate receptor subunit gene expression and stress hormone release in Lewis rats. Psychoneuroendocrinology 28: 702-714, 2003 • Makatsori A., Jezova D.: Drug-induced fetal damage and the consequences for neuroendocrine function. Pediatric Endocrinology vol. 2: 53-61, 2003 • Jezova D., Skultetyova I., Makatsori A., Moncek F., Duncko R.: Hypothalamo-pituitary-adrenocortical axis function and hedonic behavior in adult male and female rats prenatally stressed by maternal food restriction. Stress 5: 177-183, 2002 • Schwendt M., Dunčko D., Makatsori A., Johansson B.B., Ježová D.:Involvment of glutamate neurotransmission in the development of excessive wheel running in Lewis rats. Neurochemical Research ,28: 653-657,2003 • Bakos J., Duncko R., Makatsori A., Pirnik Z., Kiss A., Jezova D.: Prenatal immune challenge affects growth, behavior, and brain dopamine in the offspring. Annals of the New York Academy of Sciences (in press, 2004) • Pistovcakova J., Makatsori A., Sulcova A., Jezova D.: Felbamate reduces hormone release and locomotor hypoactivity induced by repeated stress of social defeat in mice.EuropeanNeuropsychopharmacology (in submission) • Makatsori A., Dubovicky M., Ujhazy E., Duncko R., Bakos J., Jezova D.: Neuroendocrine changes in adult female rats prenatally exposed to phenytoin. Toxicology and Applied Pharmacology (in submission) • Jezova D., Makatsori A., Duncko R., Moncek F., Jakubek M.: High trait anxiety in healthy subjects: association with low neuroendocrine responses during psychosocial stress. Progress in Neuro-psychopharmacology and Biological Psychiatry (in submission)

20. obtaining high quality scientific data with relevance to clinical research • significant improvement of the training process of young researches both from EU and Slovakia • active and broad cooperation among European scientists

22. X. International Congress of the Czech and Slovak Neurochemical Society „NEUROSCIENCE IN THE THIRD MILLENNIUM“ June 1 – 5, 2002, ČASTÁ, SLOVAKIA Institute of Experimental Endocrinology Centre of Excellence supported by European Commission Bratislava, Slovakia www.neuromeeting2002.savba.sk

23. Organised by INSTITUTE OF EXPERIMENTAL ENDOCRINOLOGY Centre of Excellence of European Commission, Bratislava, Slovakia Slovak Academy of Sciences June 28 - July 3, 2003   Smolenice Castle, Slovakia

24. Work package number and title: WP 14 Twinning and Networking with successful Centres of Excellence Objectives originally planned -          To increase the cooperation among scientists in the Central European region. -          To increase the scientific quality of meetings and training programs organized by successful Centres and other European laboratories

26. Change in the quality of theCentre The Centre • has changed into an institution that has more foreign scientists involved in local research than the number of local researchers working abroad • has become an international centre for the education of young scientists STRESSNUTS

27. Work package number and title: WP 12 Twinning and Networking with Institute of Adaptive and Space Physiology, University of Graz,Graz, Austria Objectives originally planned -          To increase the quality of the research of the Centre in the field of renin-angiotensin systems and body fluids regulation -          To apply the experience of the twinning laboratory in the research programme of the Centre

28. Twinning Laboratory Institute of Adaptive and Space Physiology and Department of Physiology, University of Graz, Austria

30. Publications and papers Hinghofer-Szalkay H., Loder I., Pilz K., Rossler A., Jezova D. : Gravity and cardiovascular regulation: Combined stimulation paradigm. Journal of Gravitational Physiology 10: 89-90, 2003 Makatsori A., Duncko R., Moncek F., Loder I., Katina S., Jezova D. : Modulation of neuroendocrine response and non-verbal behavior during psychosocial stress in healthy volunteers by the glutamate release-inhibiting drug lamotrigine. Neuroendocrinology 79: 34-42, 2004 Hinghofer-Szalkay H, Haditsch B, Loder I, Pilz K, Rössler A, Jezova D. Head down tilt at –6° to –24° can neutralize the cardiovascular effects of LBNP at –15 or –35 mmHg. Aviat Space Environ Med 2004; 75: 947-51 Hinghofer-Szalkay HG, László Z, Passath H, Pilz K, Rössler A, Jezova D., Scharfetter H.: Sodium intake does not influence bioimpedance.derived extracellular volume loss in head-down rest. Aviat. Space Environ. Med. 75: 1036-41, 2004 Loder I., Roessler A., Wurzinger G., Duncko R., Jezova D., Hinghofer-Szalkay H.: Adrenomedullin and elements of orthostatic competence after 41 hours water submersion in men. Life Sci. 2004

31. Stress model Stress model relaxing phase anticipation 15 min relaxing phase speech 15 min

32. Postural changes during public speech tests contribute to the stress response Martin Mlynarik a, Aikaterini Makatsori a, Iveta Matyiova a, Helmut G. Hinghofer-Szalkay b,Daniela Jezova a,* a Laboratory of Pharmacological Neuroendocrinology, Institute of Experimental Endocrinology, Slovak Academy of Sciences, Vlarska 3, Bratislava 83306, Slovakia b Institute for Adaptive and Spaceflight Physiology, Wormgasse 9, A-8010 Graz, Austria Running head: Postural changes and stress response