Lactase- The enzyme that would cry over spilt milk - PowerPoint PPT Presentation

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Lactase- The enzyme that would cry over spilt milk

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  1. Lactase- The enzyme that would cry over spilt milk

  2. What is lactase? • Enzyme responsible for breaking down the disaccharide lactose into the monosaccharides glucose and galactose

  3. Where do we have lactase? • Lactase is found in the villi of our small intestines

  4. Optimum Temp & pH • Optimum pH: 6 • Optimum temperature: 25oC

  5. Lactase deficiency • In humans, lactase is encoded by the LCT gene • A deficiency in this gene causes lactose intolerance • Lactase pills are available commercially • Commercial lactase is extracted from yeast and fungi

  6. Other use of Lactase • Lactase is also used to make ice cream • Adding lactase makes ice cream taste sweeter • Lactase crystallizes at low temps but the glucose and galactose remain dissolved giving ice cream a smoother texture

  7. Your mission! • Design an experiment that measures lactase activity!!! • What is available to you? • Three brands of lactase • Three different solutions • Milk • Sucrose solution • Water • Benedict’s Solution • When this solution is incubated at 95oC, glucose with turn the solution a tan/red color • Blue or white colors mean no glucose

  8. Data/Results/Conclusion • Your group should create a table that will show the color of each of your test tubes after incubation with Benedict’s solution and whether that color means the enzyme was active or not for data • Results should be a short paragraph describing your data- what worked best? What worked least? • Conclusion must discuss your results in context of your hypothesis. Discuss two things that you learned from this lab and come up with a new genetics based treatment for lactase deficiency