1 / 3

MPP OUTSIDE REQUEST FORM 12/1/10 Requestor(s ): Elizabeth Craig

MPP OUTSIDE REQUEST FORM 12/1/10 Requestor(s ): Elizabeth Craig Institution: UW Biochemistry Request Title: yeast mitochondrial Nfu1. Sequence alignment and domain structure of Nfu1p of S. cerevisiae. 26. Signal sequence. 1. 122. FeS binding site.

kapila
Download Presentation

MPP OUTSIDE REQUEST FORM 12/1/10 Requestor(s ): Elizabeth Craig

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. MPP OUTSIDE REQUEST FORM 12/1/10 Requestor(s): Elizabeth Craig Institution: UW Biochemistry Request Title: yeast mitochondrial Nfu1

  2. Sequence alignment and domain structure of Nfu1p of S. cerevisiae 26 Signal sequence 1 122 FeS binding site 243 256 : N-terminal domain, 22-123 aa. (Human NFU N-termnal domain: structured domain) : Nfu-like domain, 148-230 aa. (Human NFU C-teminal domain: molten globule domain, flexible)

  3. 10/22/11 Update – Our partner the NorthEast Structural Genomics consortium (NESG) has purified many samples of S. cereviseae Nfu1 truncations and obtained good NMR HSQC spectra on some of them, making a structure or structures in the near future quite likely. The NESG code for yeast Nfu1 is YR313; domains are named YR313A, B, etc, and series of truncations around those domains were constructed. The most successful are: YR313A, residues 17-124: ~137 mg purified, labeled with 15N/13C-5%, good HSQC obtained YR313A, residues 24-130, ~89 mg purified, labeled with 15N/13C-5%, good HSQC obtained Full-length protein has also expressed well and solubly

More Related