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Effects of MK-0457 and Vorinostat on Cell Growth and Apoptosis in MDA-MB-468 and BT-474 Cells

Study on the impact of different concentrations of MK-0457 and vorinostat on cell growth, DNA content, and apoptosis in MDA-MB-468 and BT-474 breast cancer cell lines. Results analyzed using various markers.

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Effects of MK-0457 and Vorinostat on Cell Growth and Apoptosis in MDA-MB-468 and BT-474 Cells

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  1. 30 MDA-MB-468 MDA-MB-231 25 20 % of cells with >4N DNA content 15 10 5 0 0 20 50 100 250 nM, MK-0457, 48hr Online Resource 1

  2. Online Resource 2 MDA-MB-468 Aurora B Merge Alpha Tubulin Control 20 nM MK-0457 50 nM MK-0457 100 nM MK-0457 10μm

  3. 70 BT-474 cells 60 50 40 % apoptosis 30 20 10 0 Control 1 μM vorinostat 50 nM MK-0457 250 nM MK-0457 50 nM MK-0457 + 1 μM vorinostat 250 nM MK-0457 + 1 μM vorinostat Online Resource 3 BT-474 a b 0 1.0 2.0 3.0 μM, vorinostat, 8hr IP:hsp90 (rat) Aurora A BT474 Aurora B hsp90 1 μM vorinostat 20 nM MK-0457 50 nM MK-0457 20 nM MK0457 + vorinostat 50 nM MK0457 + vorinostat IB: total cell lysates Aurora A Control hsp70 pAurora A β-actin pAurora B Aurora A c Aurora B Survivin d pSer10 H3 BT-474 β-actin 1.0 0.8 0.6 CI MK-0457 Vorinostat CI (nM) (nM) 30 300 0.824 50 500 0.682 100 1000 0.853 200 2000 0.566 0.4 0.2 0 0 0.2 0.4 0.6 0.8 1.0 Fractional Effect

  4. Online Resource 4 a * p values 0.03 for 20 combo 0.001 for 50 combo 2.0 * 1.5 CI 1.0 0.5 0 0 0.2 0.4 0.6 0.8 1.0 Fractional Effect c b MDA-MB-468 MLN8237 vorinostat Fa CI (nM) (nM) 125 1000 0.385 0.882 150 1200 0.436 0.772 175 1400 0.454 0.810 200 1600 0.49 0.755 225 1800 0.5 0.804 250 2000 0.57 0.614

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