'Dna content' presentation slideshows

RADIOBIOLOGY FOR RESIDENTS 2008 Yilun Liu, Ph.D. Molecular Biology Techniques Lecture 1: Nucleic Acids

RADIOBIOLOGY FOR RESIDENTS 2008 Yilun Liu, Ph.D. Molecular Biology Techniques Lecture 1: Nucleic Acids - DNA Fingerprinting: forensic science, paternity/materity test, genetic testing for inherited disease

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XIII Cell and Tissue Kinetics

XIII Cell and Tissue Kinetics. Cell cycle Measurement of cell cycle parameters by 3 H-thymidine Measurement by flow cytometry, DNA staining and BrdU Cell cycle synchronization techniques and uses Effect of cell cycle phase on radiosensitivity

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Eukaryotic chromosomes

Eukaryotic chromosomes. Bacterial Eukaryotic DNA is in a nucleoid body DNA is in chromosomes There is one large DNA molecule There are many molecules Circular Linear. The DNA in the diploid nucleus is ~2 meters long. It is present in a nucleus that is a 1000 cubic microns.

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Eukaryotic chromosomes

Eukaryotic chromosomes. Bacterial Eukaryotic DNA is in a nucleoid body DNA is in chromosomes There is one large DNA molecule There are many molecules Circular Linear. The DNA in the diploid nucleus is ~2 meters long. It is present in a nucleus that is a 1000 cubic microns.

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Chapt. 16 Eukaryotic Cell cycle; Chapt. 17 Stem cells

Chapt. 16 Eukaryotic Cell cycle; Chapt. 17 Stem cells. Chapt. 16 Student learning outcomes : Explain basic phases of eukaryotic cell cycle Regulators, checkpoints Describe events of mitosis Regulation by MPF, phosphorylation, proteolysis Explain regulators of progression:

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Wet Lab Radiation-induced Apoptosis

Wet Lab Radiation-induced Apoptosis. Background Equipment Supplies Procedures Lab Demonstrations. Background. Definition Distinctive features of apoptosis Apoptosis vs. necrosis Different stages (detections) of apoptosis. Apoptosis.

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Anatomy and Human Biology 313-2005 Arun Dharmarajan Professor School of Anatomy & Human Biology

Anatomy and Human Biology 313-2005 Arun Dharmarajan Professor School of Anatomy & Human Biology The University of Western Australia, Perth, Australia. Relevance of Apoptosis in Health and Disease Apoptosis is a systematic and genetically programmed process.

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Side Scatter SSC (refracted/reflected light)

Flow cytometry is a technology that simultaneously measures and then analyzes multiple physical characteristics of single particles, usually cells, as they flow in a fluid stream through a beam of light.

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Introduction

Introduction. When mammalian cells are subjected to stress signals, oxygen deficiency, radiation, DNA damage, or Chemo-therapeutic drugs, p53 is activated, leading to p53 mediated induction of programmed cell death or cell cycle arrest, or both. Apoptosis.

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Jeong Dageum UST 2009/11/04

Jeong Dageum UST 2009/11/04. <Fish taxa >. <Observation and Theory>. Rapid cladogenetic Speciation. Genomic Expansion. Gene duplication Polyploidization. southdakotapolitics.blogs.com/. southdakotapolitics.blogs.com/. <Question>. <General relationship>. Poorly characterized ?.

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Rad51-deficient vertebrate cells accumulate chromosomal breaks prior to cell death

Rad51-deficient vertebrate cells accumulate chromosomal breaks prior to cell death. BE.109 Module 1 Day 6 Manuscript discussion. What is the normal function of the RAD51 gene?.

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Figure S1

siRNA:. Control . Chk1 . TdR(h): 30 1 6 16 24 30 48 . - + - + - + - + - + - + - + . Chk1 . ? H2AX . RPA34 . Cleaved casp3 . ? -actin . siRNA:. Control . Chk1 .

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Non-transformed cells

a. Cancer. Non-transformed cells. b. Cancer. Non-transformed cells.

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Cell number

HCT116+3. PARPi. Ctr. HCT116+3+5. Ctr. PARPi. Cell number. DNA content. Fig. S2.

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200

singlet. RMS. 2.36. 800. %G1. 39. %S. 23.4. 600. %G2. 24.2. # Cells. %<G1. 1.85. 400. %>G2. 11.6. 200. 0. 0. 200. 400. 600. 800. 1000. FL2-A: DNA content. HCT116-shPBK2. singlet. RMS. 2.96. 300. %G1. 17.2. %S. 13. %G2. 42.1. 200. # Cells.

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Supplemental Figure 1

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High Content Analysis (HCA)

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Supplementary Figure 7

Supplementary Figure 7. B. *. A. **. **. **. **. **. **. *. DNA content (% of control). DNA content (% of control). C. **. *. *. *. **. **. **. DNA content (% of control).

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