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Last Time 3/4/08

Last Time 3/4/08. Increase (I) concentration increase slope intercept remains unchanged. Uncompetitive Inhibition. Inhibition only has affinity for enzyme-substrate complex Developing the rate law (1) (2) . Adding (1) and (2). From (2). Total enzyme.

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Last Time 3/4/08

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  1. Last Time 3/4/08

  2. Increase (I) concentration increase slope intercept remains unchanged.

  3. Uncompetitive Inhibition Inhibition only has affinity for enzyme-substrate complex Developing the rate law (1) (2)

  4. Adding (1) and (2) From (2)

  5. Total enzyme

  6. Slope remains the same but intercept changes as inhibitor concentration is increased

  7. Lecture16 Thursday 3/6/08 Enzyme Kinetics Noncompetive Inhibition Bioreactors Monod Equation Yield Coeficients Washout

  8. Bioreactors

  9. D=vo / V

  10. Noncompetitive, Bioreactors More uncompetitive inhibition Increasing I No I Only intercept changes Lineweaver-Burk Noncompetitive inhibition (Mixed) E + S E·S P E·I + S E·S·I Increasing I +I -I -I +I No I Both slope and intercept change

  11. Bioreactors Exponential growth phase Cells + substrate → product + more cells Cell growth can be modeled with enzyme equations since all cell reactors are enzyme catalyzed Lag phase stationary phase Death phase • Algorithm • Mass balance (later) • Rates sometimes Product concentration at which all metabolism ceases (93 g/L for wine production, n = ½) Substrate is used to form both products and cell maintenance (rate of substrate disappearance for maintenance

  12. 3) Stoichiometry – yield coefficients • Mass balance • Parameters: 2) Rates

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